Human alveolar macrophage function: differences between smokers and nonsmokers.
Human alveolar macrophage function: differences between smokers and nonsmokers.
复制标题
人类肺泡巨噬细胞功能：吸烟者和非吸烟者之间的差异。
DOI：
10.1002/jlb.44.5.313
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发表时间：
1988
影响因子：
5.5
通讯作者：
Ahmad,M
中科院分区：
文献类型：
--
作者：
Thomassen,MJ;Barna,BP;Wiedemann,HP;Farmer,M;Ahmad,M
从吸烟和不吸烟的正常志愿者身上获得人肺泡巨噬细胞和外周血单核细胞。用细菌脂多糖（LPS）体外培养巨噬细胞和单核细胞。这些细胞的氧化代谢反应是通过产生超氧阴离子来测量的。与非吸烟者的巨噬细胞相比，吸烟者的巨噬细胞对LPS激活的超氧阴离子反应受到抑制。吸烟者和非吸烟者的单核细胞没有差异。这些巨噬细胞和单核细胞的细胞毒性通过体外3h -胸苷释放试验对各种异体靶细胞进行评估。暴露于LPS的巨噬细胞和单核细胞具有杀瘤作用。来自非吸烟者的巨噬细胞似乎比来自吸烟者的巨噬细胞产生更大的细胞毒性活性。吸烟者和非吸烟者的巨噬细胞对三种不同的致瘤细胞系都有细胞毒性，但对一种非致瘤细胞系没有细胞毒性。吸烟者和非吸烟者的单核细胞在细胞毒活性上没有差异。我们得出结论，吸烟者和非吸烟者的巨噬细胞在暴露于LPS后都可以被激活；然而，来自吸烟者的巨噬细胞可能在其反应中略有抑制。
Human alveolar macrophages and peripheral blood monocytes were obtained from smoking and nonsmoking normal volunteers. The macrophages and monocytes were incubated in vitro with bacterial lipopolysaccaride (LPS). The oxidative metabolic response of these cells was measured by superoxide anion production. Macrophages from smokers were suppressed in their superoxide anion response to LPS activation as compared to macrophages from nonsmokers. Monocytes from smokers and nonsmokers were not different. The cytotoxic properties of these macrophages and monocytes were assessed by an in vitro3H-thymidine release assay against various allogeneic target cells. Macrophages and monocytes exposed to LPS were rendered tumoricidal. Macrophages from nonsmokers appeared to generate greater cytotoxic activity than macrophages from smokers. Macrophages from both smokers and nonsmokers were cytotoxic for three different tumorigenic cell lines but not for a nontumorigenic cell line. Monocytes from smokers and nonsmokers were not different in cytotoxic activity. We conclude that macrophages from both smokers and nonsmokers can be activated after exposure to LPS; however, macrophages from smokers may be slightly suppressed in their responses.