Dentin Conditioning Protocol for Regenerative Endodontic Procedures
Dentin Conditioning Protocol for Regenerative Endodontic Procedures
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DOI：
10.1016/j.joen.2020.05.010
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发表时间：
2020-08-01
影响因子：
4.2
通讯作者：
Azim, Adham A.
中科院分区：
文献类型：
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作者：
Aksel, Hacer;Albanyan, Hajar;Azim, Adham A.
本研究主要研究了次氯酸钠-EDTA灌洗对牙髓干细胞（DPSCs）活力和形态的影响，以及优化后的EDTA单独或与纳米泡（NB）水制备对细胞行为的影响。方法：在第一部分中，采用以下方案对人牙本质盘进行条件调节：(1)次氯酸钠加磷酸盐缓冲盐水（PBS），(2)第1组灌洗方案加EDTA，(3)第2组灌洗方案加PBS，(4)次氯酸钠加EDTA，(5)第4组灌洗方案加PBS。测定DPSC活力和形态。在第二部分中，采用(1)第一部分的优化方案，(2)用NB水制备EDTA，(3)超声波活化EDTA，或(4)用NB水制备的超声波活化EDTA，对牙本质盘进行调节。分别采用酶联免疫吸附法、水溶性四氮唑盐-1细胞活力法、活死法和transwell迁移法测定转化生长因子β释放和DPSC活力、形态和迁移。数据分析采用Kruskal-Wallis或单向方差分析和事后检验。结果：细胞活力以3组最高，5组次之(P
Introduction: This study focused on the optimization of sodium hypochlorite-EDTA irrigation in terms of the viability and morphology of dental pulp stem cells (DPSCs) and the effects of an optimized EDTA protocol alone or prepared with nanobubble (NB) water on cell behavior. Methods: In the first part, human dentin discs were conditioned with the following protocols: (1) Sodium hypochlorite followed by phosphate-buffered saline (PBS), (2) Irrigation protocol from group 1 followed by EDTA, (3) Irrigation protocol from group 2 followed by PBS, (4) Sodium hypochlorite followed by EDTA, (5) Irrigation protocol from group 4 followed by PBS. DPSC viability and morphology were determined. In the second part, dentin discs were conditioned with the (1) optimized protocol in the first part, (2) EDTA prepared using NB water, (3) ultrasonic-activated EDTA, or (4) ultrasonicactivated EDTA prepared using NB water. Transforming growth factor beta release and DPSC viability, morphology, and migration were determined using the enzyme-linked immunosorbent assay, the water-soluble tetrazolium salt-1 cell viability assay and live-dead assay, and the transwell migration assay, respectively. Data were analyzed using Kruskal-Wallis or one-way analysis of variance andpost hoc tests. Results: The highest cell viabilitywas observed in group 3 followed by group 5 (P