Preventative effects of lactulose in the trinitrobenzenesulphonic acid model of rat colitis
Preventative effects of lactulose in the trinitrobenzenesulphonic acid model of rat colitis
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DOI：
10.1097/01.mib.0000160808.30988.d9
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发表时间：
2005-03-01
影响因子：
4.9
通讯作者：
Galvez, J
中科院分区：
文献类型：
--
作者：
Camuesco, D;Peran, L;Galvez, J
目的：乳果糖是一种用作泻药的药物，已被证明可促进乳酸杆菌和双歧杆菌的生长，作为益生元，对炎症性肠病具有潜在的有益作用。本研究描述了乳果糖在三硝基苯磺酸（TNBS）大鼠colis.Methods模型中的预防性抗炎活性：通过结肠滴注溶于0.25 mL 50%乙醇中的10 mg TNBS使大鼠结肠炎。一组结肠炎大鼠接受乳果糖，其在TNBS滴注前掺入饮用水（2.5%wt/vol）中2周，并在结肠炎诱导后1周评价结肠损伤。检测结肠炎症的不同生化标志物：髓过氧化物酶活性、谷胱甘肽含量、肿瘤坏死因子α、白三烯134水平和结肠诱导型一氧化氮合酶表达。此外，细菌计数（乳酸杆菌和双歧杆菌）进行结肠内容物从colitic rats.Results：结果表明，乳果糖产生预防性抗炎作用，在此模型中的大鼠结肠炎，证明了髓过氧化物酶活性的显着减少，结肠肿瘤坏死因子和白三烯13，生产的减少。这种作用的特征还在于抑制结肠诱导型一氧化氮合酶的表达，这是不受调节的炎症状态的结果。这种有益的效果是与乳酸杆菌和双歧杆菌物种在结肠内容物中的水平增加，与未经处理的colitic rats.Conclusion：总之，乳果糖的肠道益生菌的影响可能与其益生元特性，支持其潜在的用途在人类炎症性肠病。
Aims: Lactulose is a drug used as a laxative that has been shown to promote the growth of lactobacilli and bifidobacteria, acting as a prebiotic and with a potential beneficial effect in inflammatory bowel disease. The present study describes the preventive antimflammatory activity of lactulose in the trinitroberizenesulphonic acid (TNBS) model of rat colitis.Methods: Rats were rendered colitic by a colonic instillation of 10 mg of TNBS dissolved in 0.25 mL of 50% ethanol. One group of colitic rats received lactulose, which was incorporated in the drinking water (2.5% wt/vol) for 2 weeks before TNBS instillation, and colonic damage was evaluated I week after colitis induction. Different biochemical markers of colonic inflammation were assayed: myeloperoxidase activity, glutathione content, tumor necrosis factor alpha, leukotriene 134 levels, and colonic inducible nitric oxide synthase expression. In addition, bacterial counts (for lactobacilli and bifidobacteria) were performed in colonic contents from colitic rats.Results: The results show that lactulose exerted a preventive anti inflammatory effect in this model of rat colitis, as evidenced by a significant reduction of myeloperoxidase activity and by a decrease of both colonic tumor necrosis factor a and leukotriene 13, production. This effect was also characterized by an inhibition of colonic inducible nitric oxide synthase expression, which is unregulated as a consequence of the inflammatory status. This beneficial effect was associated with increased levels of lactobacilli and bifidobacteria species in colonic contents in comparison with untreated colitic rats.Conclusion: In conclusion, the intestinal antiinflammatory effect of lactulose could be related to its prebiotic properties, supporting its potential use in human inflammatory bowel disease.