Global expression profiling applied to the analysis of Arabidopsis stamen development
Global expression profiling applied to the analysis of Arabidopsis stamen development
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DOI：
10.1104/pp.107.104422
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发表时间：
2007-11-01
期刊：
影响因子：
7.4
通讯作者：
Meyerowitz, Elliot M.
中科院分区：
文献类型：
--
作者：
Alves-Ferreira, Marcio;Wellmer, Frank;Meyerowitz, Elliot M.
为了获得拟南芥（Arabidopsis thaliana）雄蕊发育过程中基因表达的详细信息，我们通过微阵列分析，将野生型花序的基因表达谱与花突变体apetala3、sporatoryless/喷嘴和雄性不育1（ms1）的基因表达谱进行比较，其中雄蕊形成的不同方面被破坏。这些实验导致鉴定了在雄蕊发育的早期、中期和晚期具有预测表达的基因组。使用原位杂交的验证实验证实了预测的表达模式。其他实验旨在表征小孢子形成过程中的基因表达。为此，我们将不同发育阶段的野生型花的基因表达谱与 ms1 突变体的基因表达谱进行了比较。对来自该实验的数据集的计算分析导致了可能参与小孢子发生过程中关键发育过程控制的基因的鉴定。我们还鉴定出大量基因，与野生型相比，它们在 ms1 突变花中的表达时间延长。这一结果表明，编码假定转录调节因子的 MS1 参与了这些基因的阶段特异性抑制。最后，我们应用反向遗传学来表征微阵列实验中鉴定的几个基因，并发现了小孢子发生的新调节因子，包括转录因子 MYB99 和假定的磷脂酰肌醇 4-激酶。
To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild- type inflorescences to those of the floral mutants apetala3, sporocyteless/ nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild- type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage- specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4- kinase.