Identification and Expression of Inward-Rectifying Potassium Channel Subunits inPlutella xylostella
Identification and Expression of Inward-Rectifying Potassium Channel Subunits inPlutella xylostella
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小菜蛾内向整流钾通道亚基的鉴定与表达
DOI：
10.3390/insects11080461
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发表时间：
2020-08-01
期刊：
影响因子：
3
通讯作者：
Zhou，Xiaomao
中科院分区：
文献类型：
--
作者：
Lai，Xiaoyi;Xu，Jie;Zhou，Xiaomao
在昆虫中，内向整流钾 (Kir) 通道调节重要的生理功能，例如摄食行为、丝分泌、肾脏排泄和免疫功能。因此，它们作为杀虫剂的靶标具有广阔的潜力。双翅目和半翅目中已鉴定出三种类型的Kir亚基，但鳞翅目中的Kir亚基仍不清楚。本研究在小菜蛾转录组中鉴定出 5 个 Kir 亚基基因（pxkir1、pxkir2、pxkir3A、pxkir3B 和 pxkir4）。系统发育分析确定 pxkir1、pxkir2、pxkir3A 和 pxkir3B 是其他昆虫中 kir1-3 的直系同源基因。有趣的是，pxkir4 可能编码鳞翅目中一类新的 Kir 亚基，但迄今为止尚未有报道。为了进一步鉴定小菜蛾的 Kir 通道亚基，通过实时定量 PCR 研究了 5 个 pxkir 基因的基因表达谱。这些 pxkir 基因在小菜蛾的整个发育过程中表达。 pxkir1 和 pxkir2 在胸部和腿部高表达，而 pxkir3（3A 和 3B）和 pxkir4 在中肠和马氏小管中高表达。本研究首次鉴定了小菜蛾Kir亚基的组成和分布，为鳞翅目Kir通道亚基的进一步研究提供了有用的信息。
In insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Diptera and Hemiptera, but the Kir subunits of Lepidoptera still remain unclear. This study identified five Kir subunit genes (pxkir1, pxkir2, pxkir3A, pxkir3B, and pxkir4) in the transcriptome of Plutella xylostella. Phylogenetic analysis identified pxkir1, pxkir2, pxkir3A, and pxkir3B as orthologous genes of kir1–3 in other insects. Interestingly, pxkir4 may be encoding a new class of Kir subunit in Lepidoptera that has not been reported to date. To identify further Kir channel subunits of P. xylostella, the gene expression profiles of five pxkir genes were studied by quantitative real-time PCR. These pxkir genes are expressed throughout the development of P. xylostella. pxkir1 and pxkir2 were highly expressed in thoraxes and legs, while pxkir3 (3A and 3B) and pxkir4 had high expression levels in the midgut and Malpighian tubules. This study identified the composition and distribution of Kir subunits in P. xylostella for the first time, and provides useful information for the further study of Kir channel subunits in Lepidoptera.