Synergistic induction of cardiomyocyte differentiation from human bone marrow mesenchymal stem cells by interleukin 1β and 5-azacytidine
Synergistic induction of cardiomyocyte differentiation from human bone marrow mesenchymal stem cells by interleukin 1β and 5-azacytidine
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DOI：
10.1515/hsz-2016-0151
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发表时间：
2016-12-01
影响因子：
3.7
通讯作者：
Kianmehr, Anvarsadat
中科院分区：
文献类型：
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作者：
Khajeniazi, Safoura;Solati, Mahin;Kianmehr, Anvarsadat
白细胞介素-1 β（IL-1 β）是心肌损伤相关炎症中由心肌细胞表达的细胞因子蛋白。虽然目前有几种方法可用于治疗心脏病，但没有一种是完全成功的。近年来，干细胞受到了极大的关注，并有望在治疗心脏病方面发挥重要作用。5-最近发现氮杂胞苷（5-aza）可刺激干细胞分化为心肌细胞。在这里，我们提出了确定是否IL-1 β可以诱导间充质干细胞（MSC）分化为心肌细胞。MSC来源于骨髓，增殖，然后在补充有5-aza和IL-1 β（两个水平，5和10 ng/ml）的分化培养基中培养。21天后，提取总RNA并进行cDNA合成。定量聚合酶链反应（Q-PCR）检测心脏特异性标志物。此外，通过特异性抗体的免疫细胞化学染色，从蛋白水平研究心肌标志物的表达。最终，心脏标志物表达表明IL-1 β和5-aza协同诱导心肌细胞分化。
Interleukin-1 beta (IL-1 beta) is a cytokine protein expressed by cardiomyocyte in myocardial damage-associated inflammation. Although several methods are currently available for treatment of heart diseases none of them are completely successful. Recently, stem cells have gained enormous attention and are expected to play a significant role for treating heart diseases. 5-Azacytidine (5-aza) has recently been found to cause stimulation of stem cells to differentiate into cardiomyocytes. Here we present the determination of whether IL-1 beta can induce the differentiation of mesenchymal stem cells (MSCs) to cardiomyocytes. MSCs were derived from bone marrow, propagated and then cultured in differentiation medium supplemented with 5-aza and IL-1 beta (at two levels, 5 and 10 ng/ml). After 21 days, total RNA was extracted and cDNA synthesis was carried out. Quantitative polymerase chain reaction (Q-PCR) was performed for detecting cardiac-specific markers. Besides, to investigate the expression of cardiac markers in protein levels, immunocytochemistry was done by specific antibodies. Ultimately, cardiac markers expression suggested that IL-1 beta and 5-aza synergistically induces the cardiomyocyte differentiation.