Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair.
Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair.
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DOI：
10.1016/j.chroma.2007.06.017
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
H. Miyaguchi;M. Kakuta;Y. Iwata;H. Matsuda;Hidekatsu Tazawa;H. Kimura;H. Inoue
中科院分区：
文献类型：
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作者：
H. Miyaguchi;M. Kakuta;Y. Iwata;H. Matsuda;Hidekatsu Tazawa;H. Kimura;H. Inoue
建立了人发中甲基苯丙胺及其主要代谢物苯丙胺的高效液相色谱-串联质谱（HPLC-MS/MS）快速前处理方法，用于快速筛查和定量。将两毫克头发与100μl含10%乙腈、100 mM三氟乙酸和相应氘类似物的水溶剂一起在2 ml塑料管中机械微粉化5 min作为内标。粉碎高度分解头发成分，同时允许提取头发中存在的任何药物。在用膜过滤器单元过滤悬浮液后，通过HPLC-MS/MS直接分析澄清滤液。样品制备不需要蒸发过程。方法优化和验证研究分别使用真实情况下的样品和药物已被人工吸收的强化样品。甲基苯丙胺和苯丙胺的定量浓度范围分别为0.040-125和0.040- 25纳克/毫克。本文提出的方法和传统的方法对实际工程进行了分析，以验证本文方法对实际工程分析的适用性。我们的方法需要不到30分钟的一组色谱从洗过的头发样品获得。
We developed a rapid sample preparation method for the toxicological analysis of methamphetamine and amphetamine (the major metabolite of methamphetamine) in human hair by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS), to facilitate fast screening and quantitation. Two milligrams of hair were mechanically micropulverized for 5min in a 2-ml plastic tube together with 100μl of an aqueous solvent containing 10% acetonitrile, 100mM trifluoroacetic acid and the corresponding deuterium analogues as internal standards. The pulverizing highly disintegrated the hair components, simultaneously allowing the extraction of any drugs present in the hair. After filtering the suspension with a membrane-filter unit, the clear filtrate was directly analyzed by HPLC–MS/MS. No evaporation processes were required for sample preparation. Method optimization and validation study were carried out using real-case specimens and fortified samples in which the drugs had been artificially absorbed, respectively. Concentration ranges for quantitation were 0.040–125 and 0.040–25ng/mg for methamphetamine and amphetamine, respectively. Real-case specimens were analyzed by the method presented here and by conventional ones to verify the applicability of our method to real-world analysis. Our method took less than 30min for a set of chromatograms to be obtained from a washed hair sample.