Simultaneous Study of Mechanical Stretch-Induced Cell Proliferation and Apoptosis on C2C12 Myoblasts
Simultaneous Study of Mechanical Stretch-Induced Cell Proliferation and Apoptosis on C2C12 Myoblasts
复制标题
DOI：
10.1159/000490239
复制
发表时间：
2018-01-01
影响因子：
2.7
通讯作者：
Shi, Reng-Fei
中科院分区：
文献类型：
--
作者：
Feng, Yu;Tian, Xiang-Yang;Shi, Reng-Fei
机械牵张可导致成肌细胞增殖或凋亡。识别将拉伸细胞的命运从增殖切换到凋亡的分子事件在再生医学领域中具有实际重要性。最近的一项关于血管平滑肌细胞的研究表明，这些事件的识别可以通过在一个单一的调查中同时解决拉伸诱导的相反细胞结果来实现。为了定义可以同时研究增殖和凋亡的条件或模型，我们将体外培养的C2C12成肌细胞暴露于15%伸长率的循环机械拉伸方案，拉伸频率为1 Hz，每天0，2，4，6或8 h，连续3天。同时观察到细胞增殖和凋亡。此外，随着拉伸持续时间的延长，细胞增殖增加，直到在最佳拉伸持续时间达到峰值。之后，细胞凋亡逐渐盛行。因此，我们建立了一个模型，其中牵张诱导的细胞增殖和凋亡可以同时进行研究。(C)2018 S. Karger AG，巴塞尔
Mechanical stretch may cause myoblasts to either proliferate or undergo apoptosis. Identifying the molecular events that switch the fate of a stretched cell from proliferation to apoptosis is practically important in the field of regenerative medicine. A recent study on vascular smooth muscle cells illustrated that identification of these events may be achieved by addressing the stretch-induced opposite cellular outcomes simultaneously within a single investigation. To define conditions or a model in which both proliferation and apoptosis can be studied at the same time, we exposed in vitro cultured C2C12 myoblasts to a cyclic mechanical stretch regimen of 15% elongation at a stretching frequency of 1 Hz for 0, 2, 4, 6, or 8 h every day, consecutively, for 3 days. Both proliferation and apoptosis were observed. Moreover, as the duration of the stretch was prolonged, cell proliferation increased until it peaked at the optimal stretching duration. Afterwards, apoptosis gradually prevailed. Therefore, we established a model in which stretch-induced cell proliferation and apoptosis can be studied simultaneously. (C) 2018 S. Karger AG, Basel