Nile red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry
Nile red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry
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DOI：
10.1021/ac0517646
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发表时间：
2006-03-01
影响因子：
7.4
通讯作者：
Chang, HT
中科院分区：
文献类型：
--
作者：
Huang, YF;Chang, HT
利用尼罗红吸附的金纳米粒子(NRAuNPs)作为选择性探针和基质，建立了表面辅助激光解吸电离质谱仪(SALDI-MS)测定氨基硫醇的方法。三种氨基硫醇-谷胱甘肽(GSH)、半胱氨酸(Cys)和同型半胱氨酸(HCys)-与这些NRAuNPs表面的结合导致它们聚集，从而导致它们的颜色和荧光的后续变化。由于精氨酸-一种非硫醇氨基酸-不会导致这种聚集，因此使用NRAuNPs从包含所有四种分析物的溶液中选择性地浓缩氨基硫醇是一个简单的过程；我们能够直接通过Saldi-MS测量来识别沉淀物中的三种氨基硫醇和上清液中的精氨酸。在不使用这种预富集方法的情况下，在信噪比为3的情况下，GSH、Cys和HCys的检测限(LOD)分别为1.0、2.0和1.3微米。相比之下，使用NRAuNPs的选择性浓缩分别提供了25、54和34 nm的LOD，用于GSH、Cys和HCys的测定。与传统有机基质(如2，5-二羟基苯甲酸)相比，NRAuNP基质具有许多优点，例如制备容易、选择性、灵敏度和重复性。我们通过对红细胞中GSH和血浆中Cys的分析，验证了该方法的适用性；我们相信，该方法在诊断方面具有很大的潜力。
This paper describes the use of Nile Red-adsorbed gold nanoparticles (NRAuNPs) as selective probes and matrixes for the determination of aminothiols through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The binding of three aminothiols-glutathione (GSH), cysteine (Cys), and homocysteine (HCys)-to the surfaces of these NRAuNPs induces their aggregation, which causes asubsequent changes in their color and fluorescence. Because arginine-a non-thiol amino acid-does not induce such aggregation, it is a straightforward process to use the NRAuNPs to selectively concentrate the aminothiols from a solution containing all four of these analytes; we were able to identify the three aminothiols in the precipitate, and arginine in the supernatant, directly through SALDI-MS measurements. Without using this preconcentration approach, the limits of detection (LODs) at a signal-to-noise ratio of 3 were 1.0, 2.0, and 1.3 mu M for GSH, Cys, and HCys, respectively. In comparison, selective concentration using the NRAuNPs provided LODs of 25, 54, and 34 nM, for the determinations of GSH, Cys, and HCys, respectively. NRAuNP matrixes provide a number of advantages over the use of conventional organic matrixes (e.g., 2,5-dihydroxybenzoic acid), such as ease of preparation, selectivity, sensitivity, and repeatability. We validated the applicability of our method through the analyses of GSH in red blood cells and of Cys in plasma; we believe that this approach has great potential for diagnosis.