The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone
The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone
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DOI：
10.1302/0301-620x.90b7.20235
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发表时间：
2008-07-01
影响因子：
--
通讯作者：
Ishiguro, N.
中科院分区：
文献类型：
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作者：
Kawasumi, M.;Kitoh, H.;Ishiguro, N.
本研究旨在探讨富血小板血浆对大鼠骨髓细胞增殖和分化的影响，并确定用于骨组织工程的最佳血浆血小板浓度。将大鼠骨髓细胞包埋在不同浓度的富血小板血浆凝胶中培养6天。分析了其增殖和成骨分化的潜力。采用大鼠肢体延长模型，将培养的具有不同浓度富血小板血浆的大鼠骨髓细胞移植到牵张间隙内，影像学评价再生骨的质量。在所有富血小板血浆组中，细胞增殖以剂量依赖的方式增强。虽然各组之间碱性磷酸酶的产生和mRNA表达没有显著差异，但成熟骨再生在血小板浓度最高的组中更为普遍。我们的研究结果表明，富血小板血浆中的高血小板浓度与成骨细胞结合可以加速肢体延长过程中新骨的形成。
The aim of our study was to investigate the effect of platelet-rich plasma on the proliferation and differentiation of rat bone-marrow cells and to determine an optimal platelet concentration in plasma for osseous tissue engineering. Rat bone-marrow cells embedded in different concentrations of platelet-rich plasma gel were cultured for six days. Their potential for proliferation and osteogenic differentiation was analysed. Using a rat limb-lengthening model, the cultured rat bone-marrow cells with platelet-rich plasma of variable concentrations were transplanted into the distraction gap and the quality of the regenerate bone was evaluated radiologically.Cellular proliferation was enhanced in all the platelet-rich plasma groups in a dose-dependent manner. Although no significant differences in the production and mRNA expression of alkaline phosphatase were detected among these groups, mature bone regenerates were more prevalent in the group with the highest concentration of platelets.Our results indicate that a high platelet concentration in the platelet-rich plasma in combination with osteoblastic cells could accelerate the formation of new bone during limb-lengthening procedures.