SENSITIVITY OF A NEOPLASTIC EPITHELIAL DUCT CELL-LINE FROM A HUMAN SUB-MANDIBULAR SALIVARY-GLAND TO HUMAN-LEUKOCYTE INTERFERON AS ASSESSED BY AN INVITRO SEMISOLID AGAR TECHNIQUE
SENSITIVITY OF A NEOPLASTIC EPITHELIAL DUCT CELL-LINE FROM A HUMAN SUB-MANDIBULAR SALIVARY-GLAND TO HUMAN-LEUKOCYTE INTERFERON AS ASSESSED BY AN INVITRO SEMISOLID AGAR TECHNIQUE
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DOI：
10.1016/s0300-9785(82)80006-9
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
URATA, M
中科院分区：
文献类型：
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作者：
SATO, M;YOSHIDA, H;URATA, M
通过体外半固体琼脂技术测定来自人下颂唾液腺的肿瘤上皮导管细胞系HSG对人白细胞干扰素（HuIFN-α）的敏感性。从HSG细胞的集落中分离出在琼脂培养基中显示出最稳定生长和最大集落形成能力的克隆并用于本研究。该克隆被命名为HSG-1。结果，在含有HuIFN-α的琼脂培养基中培养HSG-1细胞，1000 U/ml的HSG-Ⅰ细胞的集落形成抑制率为47.6%。加入浓度递增的HuIFN-α。（100 U/ml ~ 1000 U/ml）对菌落数的抑制率为5.2%~ 47.6%。加入HuIFN-α。对HSG-1细胞的增殖有抑制作用。HuIFN-.alpha.可能对HSG-1细胞有抑制增殖作用。
A neoplastic epithelial duct cell line from a human submandibular salivary gland, HSG, was assayed by an in vitro semi-solid agar technique for sensitivity to human leukocyte interferon (HuIFN-.alpha.). The clone which showed the most stable growth and the greatest ability of colony formation in the agar medium was isolated from the colonies of HSG cells and used in the present study. This clone was designated as HSG-1. As a consequence, cultivation of HSG-1 cells in the agar medium containing HuIFN-.alpha. of 1000 U/ml resulted in the inhibition of 47.6% of the colony-forming ability of HSG-I cells, as compared with the untreated control. Addition of increasing concentrations of HuIFN-.alpha. (betweem 100 U/ml 1000 U/ml) in the agar medium caused a linear reduction in the number of the colonies, ranging from 5.2%-47.6%. Addition of HuIFN-.alpha. showed an inhibitory effect on the multiplication of HSG-1 cells in the monolayer culture. HuIFN-.alpha. probably has antineoplastic and antiproliferative effects on HSG-1 cells.