Glycolysis compared in intact, permeabilized and sonicated L‐929 cells
Glycolysis compared in intact, permeabilized and sonicated L‐929 cells
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完整、透化和超声处理的 L-929 细胞中糖酵解的比较
DOI：
10.1016/0014-5793(90)80192-l
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
J. Clegg
中科院分区：
文献类型：
--
作者：
S. A. Jackson;M. Thomson;J. Clegg
在完整、通透化和超声处理的L-929细胞悬液中，研究了孵育时间和细胞密度对糖酵解率的影响。声酸盐表现出对细胞密度的强烈依赖性和糖酵解率的明显滞后，而完整细胞没有细胞密度依赖性和线性糖酵解率。渗透的细胞表现出线性的糖酵解速率，但有时表现出对细胞密度的依赖。乳酸的产生率(30min/106个细胞的nmoL)在声酸盐中最高，在完整细胞中最低。这些结果被解释为支持先前提出的假设，即糖酵解途径的酶在完整的L-929细胞中高度组织。
The effects of incubation time and cell density on glycolytic rate were examined in suspensions of intact, permeabilized and sonicated L-929 cells. Sonicates exhibited strong dependence on cell density and a distinct lag in glycolytic rate, while intact cells showed no cell density dependence and linear glycolytic rates. Permeabilized cells exhibited linear glycolytic rates, but sometimes showed dependence on cell density. Rates of lactate production (nmol at 30 min/106cells) were highest in sonicates and lowest in intact cells. These results are interpreted as support for the previously proposed hypothesis that enzymes of the glycolytic pathway are highly organized in intact L-929 cells.