Genomic organization of the mouse AZ1 gene that encodes the protein localized to preacrosomes of spermatids
Genomic organization of the mouse AZ1 gene that encodes the protein localized to preacrosomes of spermatids
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DOI：
10.1006/geno.1996.4546
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发表时间：
1997-02-15
期刊：
影响因子：
4.4
通讯作者：
Tajima, S
中科院分区：
文献类型：
--
作者：
Aoto, H;Miyake, Y;Tajima, S
AZ1蛋白定位于精子细胞的顶体前区域。以往的发育研究表明，AZ1基因在粗线期精母细胞中开始转录，在培养的成纤维细胞中被5-氮胞苷诱导表达，从而导致基因组DNA去甲基化。在本研究中，我们克隆了编码AZ1基因的基因，并阐明了它的基因组结构。AZ1基因由25个外显子组成，外显子分布在基因组DNA的25kb内。AZ1基因的外显子1和内含子1的一部分组成了一个典型的CpG岛，5‘端约0.2kb的序列显示其G+C含量和CpG比率高于哺乳动物基因组序列的平均值。(C)1997年学术出版社。
The AZ1 protein is localized to the preacrosome region of spermatids. Previous developmental studies suggested that AZ1 gene transcription begins in pachytene spermatocytes and that expression of the gene is induced in cultivated fibroblasts on treatment with 5-azacytidine, which is known to lead to the demethylation of genomic DNA. In the present study, we cloned the gene coding AZ1 cDNA and elucidated its genomic organization. AZ1 cDNA was encoded by 25 exons distributed within 25 kb of genomic DNA. The transcription started at nucleotide positions -312 and -311 of the cDNA, with the A of the ATG codon specifying the initiator methionine assigned as nucleotide position +1. Exon 1 and a part of intron 1 of the AZ1 gene constituted a typical CpG island, and an about 0.2-kb sequence of the 5'-proximal region exhibited a G + C content and CpG ratio higher than the averages for the mammalian genomic sequence. (C) 1997 Academic Press.