Potential of Bacillus species against Meloidogyne javanica parasitizing eggplant (Solanum melongena L.) and induced biochemical changes
Potential of Bacillus species against Meloidogyne javanica parasitizing eggplant (Solanum melongena L.) and induced biochemical changes
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DOI：
10.1007/s11104-013-1931-6
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发表时间：
2014-02-01
期刊：
影响因子：
4.9
通讯作者：
Khan, D.
中科院分区：
文献类型：
--
作者：
Abbasi, Muhammad Waseem;Ahmed, Naeem;Khan, D.
研究了枯草芽孢杆菌(BS)、固性芽孢杆菌(BF)和凝结芽孢杆菌(BC)3种芽孢杆菌对茄子根结线虫的生防效果。在茄子(Solanum melongena L.)幼苗周围的土壤中接种芽孢杆菌。在温室实验中，有线虫和没有线虫。接种后15天和45天观察了茄子植株的生长、生化变化和线虫寄生情况，其中BC显著促进了茄子植株的生长，提高了叶片中的叶绿素b含量和总叶绿素含量以及多酚氧化酶(PPO)活性，而BS对根结线虫的寄生抑制作用最强。15天和45天，对照(C-)植株的叶绿素b、类胡萝卜素、可溶性蛋白含量和愈创木酚过氧化物酶活性低于感染对照(C+)，而过氧化氢酶(CAT)和多酚氧化酶(PPO)活性高于感染对照(C+)和对照(C+)。在45天时，大多数处理植株的超氧化物歧化酶活性下降，在15天时又有所上升。在C-和C+植物中，抗坏血酸过氧化物酶活性在45天比15天有所增加。在45DAI时，所有处理和对照的PAL活性都比15DAI时显著提高。BC是一种潜在的植物生长促进菌，尽管它对线虫侵染的效果不如BS。酶活性因感染和DAI不同而不同。一般情况下，15天的BC提高了大多数应激酶的活性，从而克服了线虫寄生的影响。
The biocontrol potential of three Bacillus species, namely Bacillus subtilis (BS), Bacillus firmus (BF), and Bacillus coagulans (BC) was tested against the root-knot nematode Meloidogyne javanica (Treub) Chitwood in eggplants (Solanum melongena L.). Plant growth and biochemical effects were also measured in these interactions.Bacillus species were inoculated in soil around the seedlings of eggplants (Solanum melongena L.) with and without nematodes in a greenhouse experiment. Plant growth, biochemical changes, and nematode parasitism were observed at 15 and 45 days after inoculation (DAI).BC significantly enhanced plant growth, chlorophyll "b" and total chlorophyll contents, and polyphenol oxidase (PPO) activity in the leaves of eggplants, while BS showed greatest reduction in root-knot nematode parasitism. Non-infected and untreated control (C-) plants showed lesser chlorophyll "b," carotenoids, soluble protein contents, and guaiacol peroxidase but higher catalase and PPO activities compared to infected and untreated controls (C+) at 15 and 45 DAI. Superoxide dismutase activity declined in most of the treated plants at 45 DAI following rise at 15 DAI. Ascorbate peroxidase activity increased at 45 DAI compared to 15 DAI in C- and C+ plants. PAL activity was greatly enhanced at 45 DAI in all treatments and controls over that at 15 DAI.BC is a potentially plant growth-promoting bacteria although it was less effective against nematode infection compared to BS. Enzymes activities varied with infection and DAI. BC at 15 DAI in general increased the activity of most of the stress enzymes and thereby overcoming the effect of nematode parasitism.