Hippocampal Pyramidal Neurons Switch from a Multipolar Migration Mode to a Novel "Climbing" Migration Mode during Development
Hippocampal Pyramidal Neurons Switch from a Multipolar Migration Mode to a Novel "Climbing" Migration Mode during Development
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DOI：
10.1523/jneurosci.2254-13.2014
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发表时间：
2014-01-22
影响因子：
5.3
通讯作者：
Nakajima, Kazunori
中科院分区：
文献类型：
--
作者：
Kitazawa, Ayako;Kubo, Ken-ichiro;Nakajima, Kazunori
海马体在大脑功能中发挥着重要作用。尽管海马功能很重要，但最近对神经元迁移的分析主要是在大脑新皮质上进行的，而负责海马形成的细胞机制尚未完全了解。此外，为什么海马神经元需要很长时间才能完成迁移，几十年来一直无法解释。我们分析了发育中的小鼠海马CA1区神经元的迁移特征，发现脑室附近生成的海马锥体神经元在发育后期成为有丝分裂后多极细胞并在多极细胞积累区（MAZ）中积累。海马神经元以独特的迁移方式穿过锥体层。它们的主要过程是高度分支的，并与许多径向纤维接触。延时成像显示，迁移的细胞将其支架从原来的放射状纤维改变为其他放射状纤维，因此它们以之字形方式行进，间隔很长。海马体中的迁移细胞让我们想起了“攀岩者”，他们不是用手来拉动身体，而是用主导过程来拉动细胞体。由于这种迁移模式从未被描述过，因此我们将其称为“攀爬”模式。从海马体的“攀爬”模式到新皮质的“运动”模式的转变可能有助于进化过程中大脑的扩张。
The hippocampus plays important roles in brain functions. Despite the importance of hippocampal functions, recent analyses of neuronal migration have mainly been performed on the cerebral neocortex, and the cellular mechanisms responsible for the formation of the hippocampus are not yet completely understood. Moreover, why a prolonged time is required for hippocampal neurons to complete their migration has been unexplainable for several decades. Weanalyzed the migratory profile of neurons in the developing mouse hippocampal CA1 region and found that the hippocampal pyramidal neurons generated near the ventricle became postmitotic multipolar cells and accumulated in the multipolar cell accumulation zone (MAZ) in the late stage of development. The hippocampal neurons passed through the pyramidal layer by a unique mode of migration. Their leading processes were highly branched and made contact with many radial fibers. Time-lapse imaging revealed that the migrating cells changed their scaffolds from the original radial fibers to other radial fibers, and as a result they proceed in a zigzag manner, with long intervals. The migrating cells in the hippocampus reminded us of "rock climbers" that instead of using their hands to pull up their bodies were using their leading processes to pull up their cell bodies. Because this mode of migration had never been described, we called it the "climbing" mode. The change from the "climbing" mode in the hippocampus to the "locomotion" mode in the neocortex may have contributed to the brain expansion during evolution.