Highly monodispersed gold nanoparticles synthesis and inhibition of herpes simplex virus infections
Highly monodispersed gold nanoparticles synthesis and inhibition of herpes simplex virus infections
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DOI：
10.1016/j.msec.2018.04.005
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发表时间：
2018-08-01
影响因子：
7.9
通讯作者：
Mukherjee, Arup
中科院分区：
文献类型：
--
作者：
Haider, Asim;Das, Suvadra;Mukherjee, Arup
这项工作涉及准球形金纳米颗粒的合成以及针对单纯疱疹病毒（HSV）感染的成功抗病毒功效评估。超声波诱导没食子酸 (GA) 快速减少，产生高度单分散的金纳米颗粒 (GAunps)。 GAunps 等离子体峰在 531 nm 处记录，TEM 尺寸为 7.86 nm。 X射线衍射和SAED图证明了fcc晶体结构。 FTIR 研究证实纳米粒子表面与没食子酸结合。研究了 GAunps 对 Vero 细胞中 HSV 感染的抗病毒功效。 GAunps以剂量依赖性方式有效，HSV-1中的EC50为32.3μM，HSV-2中的EC50为38.6μM。进一步的研究表明，GAunps 可以阻止病毒附着和渗透到 Vero 细胞中。抑制百分比随着纳米颗粒在感染细胞中的暴露时间而变化。纳米颗粒在 Vero 细胞中的细胞毒性 (CC50 972.4 mu M) 显着低于阿昔洛韦 (CC50 561.7 mu M)，表明其安全性。生物安全的金纳米粒子被提议作为病毒化疗中更安全的替代品。
This work relates to quasi spherical gold nanoparticles synthesis and successful antiviral efficacy evaluations against Herpes simplex virus (HSV) infections. Ultrasound induced rapid reduction in gallic acid (GA) leads to highly monodispersed gold nanoparticles (GAunps). GAunps plasmonic peak was recorded at 531 nm with TEM size of 7.86 nm. X-ray diffraction and SAED pattern proved fcc crystalline structure. FTIR studies confirmed nanoparticles surface conjugation with gallic acid. The antiviral efficacy of GAunps was studied against HSV infections in Vero cells. GAunps were effective in dose-dependent manner with EC50 of 32.3 mu M in HSV-1 and 38.6 mu M in HSV-2. Further study revealed that GAunps prevented viral attachment and penetration into the Vero cells. The inhibition percentage varied with the nanoparticles exposure time in infected cells. Nanoparticles cytotoxicity (CC50 972.4 mu M) in Vero cells was significantly lower than acyclovir (CC50 561.7 mu M) indicating its safety. Bio-safe gold nanoparticles were proposed as a safer alternative in virus chemotherapy.