Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems
Microfabricated curtains for controlled cell seeding in high throughput microfluidic systems
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DOI：
10.1039/b819622b
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Walker, Glenn M.
中科院分区：
文献类型：
--
作者：
O'Neill, Adrian T.;Monteiro-Riviere, Nancy A.;Walker, Glenn M.
提出了一种微加工细胞幕，便于细胞测定。细胞幕被定义为聚二甲基硅氧烷（PDMS）壁，从细胞培养微室的天花板延伸到室地板的微米范围内。通过观察单层人表皮角质形成细胞（HEK）菌落，证明了幕帘的使用比无幕帘的微流控室长48小时。将膜整合到96室高通量微流控细胞培养装置中进一步表征。作为概念的证明，该装置用于测定细胞培养基中0-22%的乙醇稀释度范围。细胞暴露于12%或更少的乙醇中30分钟，24小时存活率恢复到85%，而暴露于更高浓度的细胞存活率低于10%。数据还显示，暴露在6%或更少乙醇浓度下的细胞种群规模增长，8%的乙醇浓度会阻碍生长，更高的浓度会导致种群数量减少。与多孔板相比，幕帘的使用允许较高的初始细胞播种密度，并增加了细胞的培养时间。
A microfabricated cell curtain is presented that facilitates cellular assays. The cell curtain is defined as a poly(dimethylsiloxane) (PDMS) wall that extends from the ceiling of a cell culture microchamber to within microns of the chamber floor. Curtain use is demonstrated by observing monolayer human epidermal keratinocyte (HEK) colonies for 48 h longer than possible with non-curtained microfluidic chambers. The curtains were further characterized by integrating them into a 96 chamber high throughput microfluidic cell culture device. As proof of concept, this device was used to assay a range of ethanol dilutions spanning 0-22% in cell culture medium. Cells exposed to 12% ethanol or less for 30 min would recover to 85% viability at 24 h, while cells exposed to higher concentrations had viabilities below 10%. The data also showed that cells exposed to 6% ethanol or less grew in population size, 8% ethanol exposure stunted growth, and higher concentrations led to population loss. Curtain use permitted high initial cell seeding densities and increased the amount of time cells can be cultured compared to multi-well plates.