Ontogeny of local sarcoplasmic reticulum Ca2+ signals in cerebral arteries -: Ca2+ sparks as elementary physiological events
Ontogeny of local sarcoplasmic reticulum Ca2+ signals in cerebral arteries -: Ca2+ sparks as elementary physiological events
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DOI：
10.1161/01.res.83.11.1104
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发表时间：
1998-11-30
影响因子：
20.1
通讯作者：
Nelson, MT
中科院分区：
文献类型：
--
作者：
Gollasch, M;Wellman, GC;Nelson, MT
肌浆网中通过ryanodine受体（RyRs）释放Ca2+是肌肉兴奋-收缩耦合的关键因素。在动脉平滑肌中，Ca2+通过RyRs释放激活Ca2+敏感的K+ (K- ca)通道，以对抗血管收缩。在平滑肌和横纹肌中观察到局部Ca2+瞬变（“Ca2+火花”），显然是由簇状RyRs的开放引起的。我们通过检查RyRs在脑动脉个体发育过程中的功能，探讨了RyRs是否产生Ca2+火花来调节动脉平滑肌张力的基本问题。在本研究中，使用荧光Ca2+指示剂fluo-3结合激光扫描共聚焦显微镜测量Ca2+火花。直径和动脉壁[Ca2+]测量从孤立的加压动脉也用于本研究提供功能见解。新生儿动脉(
Ca2+ release through ryanodine receptors (RyRs) in the sarcoplasmic reticulum is a key element of excitation-contraction coupling in muscle. In arterial smooth muscle, Ca2+ release through RyRs activates Ca2+-sensitive K+ (K-Ca) channels to oppose vasoconstriction. Local Ca2+ transients ("Ca2+ sparks"), apparently caused by opening of clustered RyRs, have been observed in smooth and striated muscle. We explored the fundamental issue of whether RyRs generate Ca2+ sparks to regulate arterial smooth muscle tone by examining the function of RyRs during ontogeny of arteries in the brain. In the present study, Ca2+ sparks were measured using the fluorescent Ca2+ indicator fluo-3 combined with laser scanning confocal microscopy. Diameter and arterial wall [Ca2+] measurements obtained from isolated pressurized arteries were also used in this study to provide functional insights. Neonatal arteries (