Interleukin-17A Plays a Pivotal Role in Polymicrobial Sepsis According to Studies Using IL-17A Knockout Mice
Interleukin-17A Plays a Pivotal Role in Polymicrobial Sepsis According to Studies Using IL-17A Knockout Mice
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DOI：
10.1016/j.jss.2010.11.901
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发表时间：
2012-05-01
影响因子：
2.2
通讯作者：
Fujii, Hideki
中科院分区：
文献类型：
--
作者：
Ogiku, Masahito;Kono, Hiroshi;Fujii, Hideki
背景资料。白细胞介素17A(IL-17A)是一种促炎细胞因子，在中性粒细胞募集中起重要作用。我们研究IL-17A在小鼠多菌败血症模型中的作用。材料与方法。对IL-17A基因敲除小鼠(KO)和野生型(WT)小鼠进行盲肠结扎穿孔(CLP)。在CLP术后7d评估存活时间，并在CLP后12h评估组织病理学改变。CLP后12h采血进行细菌培养。采用酶联免疫吸附试验(ELISA法)检测大鼠CLP后血清中炎症介质的表达。免疫组织化学染色和流式细胞术检测FOXP3和IL-17A在小鼠脾组织中的表达。与WT小鼠相比，CLP后KO小鼠的死亡率增加。此外，KO小鼠血液中的细菌生长和血清高迁移率族蛋白1(HMGB1)水平也显著高于WT小鼠。KO小鼠脾组织中FOXP3的表达明显高于WT小鼠。IL-17A在感染性腹膜炎的宿主防御中起关键作用。(C)2012 Elsevier Inc.保留所有权利。
Background. Interleukin (IL)-17A is a proinflammatory cytokine and plays an important role in neutrophil recruitment. We investigate the role of IL-17A in a mouse polymicrobial sepsis model.Materials and Methods. IL-17A knockout mice (KO) and wild-type (WT) mice were subjected the cecal ligation and puncture (CLP). Survival was assessed for the following 7 d after the CLP operation, and histopathologic findings were evaluated 12 h after CLP. Bacterial outgrowth in blood was assessed by blood culture 12 h after CLP. After CLP, expression of inflammatory mediators in serum was assessed by enzyme-linked immunosorbent assay (ELISA). Furthermore, expression of FOXP3 and IL-17A in the spleen was assessed by immunohistochemical staining and flow cytometry.Results. Mortality was increased in KO mice compared with WT mice after CLP. Furthermore, bacterial outgrowth in blood and serum high mobility group box 1 (HMGB1) levels were also significantly greater in KO mice than WT mice. The expression of FOXP3 in the spleen was significantly greater in KO mice than WT mice.Conclusion. IL-17A play pivotal role in host defense during septic peritonitis. (C) 2012 Elsevier Inc. All rights reserved.