Antimicrobial activity of honey from the stingless bee Trigona carbonaria determined by agar diffusion, agar dilution, broth microdilution and time-kill methodology
Antimicrobial activity of honey from the stingless bee Trigona carbonaria determined by agar diffusion, agar dilution, broth microdilution and time-kill methodology
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DOI：
10.1111/j.1365-2672.2009.04552.x
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Hammer, K. A.
中科院分区：
文献类型：
--
作者：
Boorn, K. L.;Khor, Y-Y.;Hammer, K. A.
Aims:The aim of this study was to determine the spectrum of antimicrobial activity of 11 samples of stingless bee honey compared to medicinal, table and artificial honeys.Methods and Results:Activity was assessed by agar diffusion, agar dilution, broth microdilution and time-kill viability assays. By agar dilution, minimum inhibitory concentration (MIC) ranges were 4% to > 10% (w/v) for Gram-positive bacteria, 6% to > 16% (w/v) for Gram-negative bacteria and 6% to > 10% (w/v) for Candida spp. By broth microdilution, all organisms with the exception of Candida albicans and Candida glabrata were inhibited at < 32% (w/v). Geometric MIC (w/v) means for stingless bee honeys ranged from 7 center dot 1% to 16 center dot 0% and were 11 center dot 7% for medicinal honey and 26 center dot 5% for table honey. Treatment of organisms with 20% (w/v) stingless bee honey for 60 min resulted in decreases of 1-3 log for Staphylococcus aureus, > 3 log for Pseudomonas aeruginosa and < 1 log for C. albicans. Similar treatment with each control honey resulted in decreases of < 1 log for all organisms.Conclusions:Stingless bee honey has broad-spectrum antibacterial activity although activity against Candida was limited. Stingless bee honey samples varied in activity and the basis for this remains to be determined.Significance and Impact of the Study:Stingless bee honey had similar activity to medicinal honey and may therefore have a role as a medicinal agent.
