Lunatic fringe potentiates Notch signaling in the developing brain
Lunatic fringe potentiates Notch signaling in the developing brain
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DOI：
10.1016/j.mcn.2010.05.004
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发表时间：
2010-09-01
影响因子：
3.5
通讯作者：
Matsuzaki, Fumio
中科院分区：
文献类型：
--
作者：
Kato, Tomoaki M.;Kawaguchi, Ayano;Matsuzaki, Fumio
Notch信号传导对于哺乳动物神经祖细胞的自我更新是必不可少的。多种机制调节Notch信号传导以平衡祖细胞的自我更新和分化。Fringe是一种主要的Notch调节剂，并根据Notch配体促进或抑制Notch信号传导。在发育中的大脑中。Lunatic fringe（Lfng）在自我更新的祖细胞中表达，但其作用尚不清楚。在这项研究中，在体内嵌合体分析使用在子宫内电穿孔的研究Lfng在神经前体细胞中的作用。我们发现Lfng增强Notch信号传导细胞自主性。它的耗尽并不影响神经定向细胞和自我更新祖细胞之间的平衡，然而，无论细胞密度的Lfng耗尽的细胞，并没有造成明显的缺陷，在脑发育。用Notch配体进行的体内过表达实验表明，Lfng强烈增强了由Delta样1而不是Jagged 1介导的Notch信号传导。(C)2010年爱思唯尔公司All rights reserved.
Notch signaling is essential for the self-renewal of mammalian neural progenitor cells. A variety of mechanisms modulate Notch signaling to balance the self-renewal and differentiation of progenitor cells. Fringe is a major Notch regulator and promotes or suppresses Notch signaling, depending on the Notch ligands. In the developing brain. Lunatic fringe (Lfng) is expressed in self-renewing progenitors, but its roles are unknown. In this study, in vivo mosaic analyses using in utero electroporation were developed to investigate the roles of Lfng in neural progenitor cells. We found that Lfng potentiates Notch signaling cell-autonomously. Its depletion did not affect the balance between neuronally committed cells and self-renewing progenitors, however, irrespective of the cell density of Lfng-depleted cells, and caused no obvious defects in brain development. In vivo overexpression experiments with Notch ligands suggest that Lfng strongly augments Notch signaling mediated by Delta-like 1 but not Jagged 1. (C) 2010 Elsevier Inc. All rights reserved.