Recognition of B and T cell epitopes by cattle immunized with a synthetic peptide containing the major immunogenic site of VP1 FMDV 01 Campos.
Recognition of B and T cell epitopes by cattle immunized with a synthetic peptide containing the major immunogenic site of VP1 FMDV 01 Campos.
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DOI：
10.1006/viro.1994.1305
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发表时间：
1994-06
期刊：
影响因子：
3.7
通讯作者：
P. Zamorano;A. Wigdorovitz;M. Chaher;F. Fernández;Consuelo Carrillo;F. Marcovecchio;A. Sadir;M. Borca
中科院分区：
文献类型：
--
作者：
P. Zamorano;A. Wigdorovitz;M. Chaher;F. Fernández;Consuelo Carrillo;F. Marcovecchio;A. Sadir;M. Borca
B和T细胞表位的精确位置已经在含有FMDV毒株01 Campos（01 C）VP 1的主要免疫原性位点（残基135-160）的肽中建立。肽（p135-160），游离或共轭牛血清白蛋白，诱导豚鼠的完全保护和牛的强中和抗体（NAb）的反应。使用一组部分重叠的肽显示，尽管几个B细胞表位沿着p135-160分布，但负责诱导NA B的残基限于氨基酸135-144。此外，用p135-160免疫的牛显示出对该肽的强烈增殖反应，但对纯化的病毒的反应非常差。两个T-表位位于135-160序列中：一个在135-144区，另一个在150-160区。在用灭活病毒疫苗免疫的牛中证实了这两个T细胞表位的免疫优势。
The precise location of B and T cell epitopes have been established in a peptide containing the major immunogenic site (residues 135-160) of FMDV strain 01 Campos (01C) VP1. The peptide (p135-160), administered free or conjugated to bovine serum albumin, induced complete protection in guinea pigs and a strong neutralizing antibody (NAb) response in cattle. Using a set of partially overlapping peptides it was shown that although several B cell epitopes were distributed along the p135-160, the residues responsible for the induction of NAb were restricted to the amino acids 135-144. In addition, bovines immunized with the p135-160 showed a strong proliferative response to this peptide but a very poor response against purified virus. Two T-epitopes were located in the 135-160 sequence: one on the region 135-144 and the other in the region 150-160. The immunodominance of these two T cell epitopes was confirmed in cattle immunized with inactivated virus vaccines.