Genetic analysis of starch paste viscosity parameters in glutinous rice (Oryza sativa L.)
Genetic analysis of starch paste viscosity parameters in glutinous rice (Oryza sativa L.)
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糯米淀粉糊粘度参数的遗传分析（Oryza sativa L.）
DOI：
10.1007/s00122-010-1423-5
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发表时间：
2011-01-01
影响因子：
5.4
通讯作者：
Gu, Ming-Hong
中科院分区：
文献类型：
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作者：
Yan, Chang-Jie;Tian, Zhi-Xi;Gu, Ming-Hong
淀粉糊粘度是评价大米蒸煮、食用和加工品质的重要指标。糯米淀粉糊粘度的遗传尚不清楚。本研究以118份糯稻材料为材料，利用43个基因特异性分子标记对17个淀粉合成相关基因(SSRG)进行了基因分型。关联分析表明，17个SSRG中有10个参与控制快速粘度仪(RVA)的轮廓参数。其中，pul基因对糯米的峰值粘度(PKV)、热糊粘度(HPV)、冷糊粘度(CPV)、崩解粘度(BDV)、峰值时间(Pet)和糊化温度(PAT)起着重要的控制作用。其他SSRG只涉及几个RVA轮廓参数。此外，SSRG之间的相互作用被发现是导致宠物、PAT和BDV的原因。一些RVA参数，包括PKV、HPV、CPV、CSV和PAT，主要由单个SSRG控制，而其他参数，如BDV、SBV和PET，则由几个SSRG控制，共同发挥作用。此外，还获得了三个近等基因系(NIL)。以Suyunuo为遗传背景，将Pu1、SSIII-1和SSIII-2等位基因替换为籼稻品种。利用桂超2号对各基因的遗传效应进行了验证，结果与关联分析的结果一致。这些结果表明，糯米淀粉糊粘度是一个复杂的遗传系统，PUL基因在决定糯米RVA谱参数中起着重要作用。这些结果为糯米品质的潜在改良提供了重要信息。
Starch paste viscosity plays an important role in estimating the cooking, eating, and processing quality of rice. The inheritance of starch paste viscosity in glutinous rice remains undefined. In the present study, 118 glutinous rice accessions were collected, and the genotypes of 17 starch synthesis-related genes (SSRG) were analyzed by using 43 gene-specific molecular markers. Association analysis indicated that 10 of 17 SSRGs were involved in controlling the rapid visco analyzer (RVA) profile parameters. Among these, the PUL gene was identified to play an important role in control of peak viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), peak time (PeT), and paste temperature (PaT) in glutinous rice. Other SSRGs involved only a few RVA profile parameters. Furthermore, interactions between SSRGs were found being responsible for PeT, PaT, and BDV. Some of the RVA parameters, including PKV, HPV, CPV, CSV, and PaT, were mainly governed by single SSRG, whereas other parameters, such as BDV, SBV, and PeT, were controlled by a few SSRGs, functioning cooperatively. Further, three near-isogenic lines (NIL) of a japonica glutinous cv. Suyunuo as genetic background, with PUL, SSIII-1, and SSIII-2 alleles replaced with those of indica cv. Guichao 2, were employed to verify the genetic effects of the various genes, and the results were consistent with those obtained from the association analysis. These findings indicated that starch paste viscosity in glutinous rice had a complex genetic system, and the PUL gene played an important role in determining the RVA profile parameters in glutinous rice. These results provide important information for potentially improving the quality of glutinous rice.