Fourth ventricular injection of selective bombesin receptor antagonists facilitates feeding in rats.
Fourth ventricular injection of selective bombesin receptor antagonists facilitates feeding in rats.
复制标题
第四心室注射选择性铃蟾肽受体拮抗剂有利于大鼠进食。
DOI：
10.1152/ajpregu.1993.264.1.r218
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Flynn,FW
中科院分区：
文献类型：
--
作者：
Flynn,FW
外源性蛙皮素（BN）样肽通过尾侧脑干BN样肽及其受体发挥摄食作用。为了确定内源性BN样肽是否参与摄食控制并对尾侧脑干神经元发挥作用，向非剥夺大鼠第四脑室注射以下剂量的生理盐水或BN受体拮抗剂：5和25 ng [D-Phe12，Leu14] BN或5 ng [D-Phe6] BN（6 - 13）甲酯。每5分钟测量一次牛奶摄入量，每只动物的行为（进食、梳理、探索、休息）每1分钟监测一次，持续60分钟。与盐水注射相比，第四脑室注射25 ng [D-Phe12，Leu14] BN和5 ng [D-Phe6] BN（6 - 13）甲酯可靠地促进牛奶摄入。在注射后5分钟内，牛奶摄入量明显增加，并在整个1小时摄入量试验中持续存在。第四脑室注射生理盐水和促进进食的拮抗剂后的行为序列相似。注射[D-Phe 12，Leu 14] BN和[D-Phe 6] BN（6 - 13）甲酯未产生普遍性唤醒。结果表明，食物相关的，内源性BN样肽有助于终止喂养和发挥其行为控制作用的BN样肽受体附近的第四脑室。
Exogenous bombesin (BN)-like peptides exert their effects on feeding through caudal brain stem BN-like peptides and their receptors. To determine if endogenous BN-like peptides are involved in the control of feeding and exert their actions on caudal brain stem neurons, nondeprived rats were administered fourth ventricular injections of either saline or BN receptor antagonists in the following doses: 5 and 25 ng [D-Phe12,Leu14]BN or 5 ng [D-Phe6]BN(6-13)methyl ester. Milk intake was measured every 5 min and each animal's behavior (eating, grooming, exploring, resting) was monitored every 1 min for 60 min. Compared with saline injections, fourth ventricular injections of 25 ng [D-Phe12,Leu14]BN and 5 ng [D-Phe6]BN(6-13)methyl ester reliably facilitated milk intake. The enhanced milk intake was evident within 5 min after the injection and persisted throughout the 1-h intake test. The behavioral sequence following fourth ventricular injections of saline and the antagonists that facilitated feeding were similar. Injections of [D-Phe12,Leu14]BN and [D-Phe6]BN(6-13)methyl ester did not produce a generalized arousal. The results indicate that food-contingent, endogenous BN-like peptides contribute to the termination of feeding and exert their behavior-controlling actions on BN-like peptide receptors in the vicinity of the fourth ventricle.