Fimbria-specific antibodies in serum and saliva of mice immunized with Actinomyces viscosus T14V fimbriae.
Fimbria-specific antibodies in serum and saliva of mice immunized with Actinomyces viscosus T14V fimbriae.
复制标题
用粘性放线菌 T14V 菌毛免疫的小鼠血清和唾液中的菌毛特异性抗体。
DOI：
10.1128/iai.54.2.507-515.1986
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发表时间：
1986
影响因子：
3.1
通讯作者：
Clark,WB
中科院分区：
文献类型：
--
作者：
Crawford,PC;Clark,WB
在用纯化的菌毛粘附素免疫的小鼠中比较下颌下腺区域（即，本地站点）或在后面的远程站点。将100微克分离自粘性放线菌T14 V的菌毛用作疫苗。在局部部位皮下注射疫苗4次，血清和唾液中的菌毛特异性免疫球蛋白G（IgG）含量高于3次注射。然而，在血清和唾液中的菌毛特异性伊加的反应没有差异。在本地或远程站点的初次免疫后21天，首先在血清和唾液中检测到菌毛特异性IgG。在本地或远程站点的初次免疫后28天首次检测到血清中的菌毛特异性伊加。然而，唾液中的菌毛特异性伊加仅发生在用菌毛疫苗在局部部位免疫的小鼠中，并且在初次免疫后14天首次检测到。用菌毛疫苗局部免疫4次的小鼠的血清和唾液都抑制了T14 V株细胞在用正常小鼠唾液预处理的羟基磷灰石珠上的体外吸附，而悬浮在假免疫动物血清和唾液中的T14 V株细胞的吸附不受抑制。总的来说，这些数据表明，小鼠局部免疫的纯化菌毛粘附素组成的疫苗在下颌下腺区域提供了一个潜在的模型，用于评估效力的唾液中的菌毛特异性抗体抑制菌株T14 V的牙齿表面的殖民。
Fimbria-specific antibody responses were compared in mice immunized with purified fimbrial adhesins in the region of the submandibular gland (i.e., local site) or at a remote site in the back. One hundred micrograms of fimbriae isolated from Actinomyces viscosus T14V was used as the vaccine. Four subcutaneous injections of the vaccine in the local site induced greater amounts of fimbria-specific immunoglobulin G (IgG) in serum and saliva than three injections. However, there was no difference in the response of fimbria-specific IgA in serum and saliva. Fimbria-specific IgG in serum and saliva were first detected 21 days after the primary immunization at both the local or remote sites. Fimbria-specific IgA in serum was first detected 28 days after the primary immunization at both the local or remote sites. However, fimbria-specific IgA in saliva occurred only in mice immunized with the fimbrial vaccine at the local site and was first detected 14 days after the primary immunization. Both serum and saliva from mice immunized 4 times with the fimbrial vaccine in the local site inhibited in vitro adsorption of strain T14V cells to hydroxyapatite beads pretreated with normal mouse saliva, whereas adsorption of strain T14V cells suspended in serum and saliva from sham-immunized animals was not inhibited. Collectively, these data suggest that mice immunized locally in the submandibular gland region with a vaccine composed of purified fimbrial adhesins provide a potential model for evaluating the efficacy of fimbria-specific antibodies in saliva to inhibit strain T14V colonization of tooth surfaces.