Acetylglyceryl ether phosphorylcholine (AGEPC; platelet-activating factor)-induced stimulation of rabbit platelets: correlation between phosphatidic acid level, 45Ca2+ uptake, and [3H]serotonin secretion.
Acetylglyceryl ether phosphorylcholine (AGEPC; platelet-activating factor)-induced stimulation of rabbit platelets: correlation between phosphatidic acid level, 45Ca2+ uptake, and [3H]serotonin secretion.
复制标题
乙酰甘油醚磷酸胆碱（AGEPC；血小板激活因子）诱导的兔血小板刺激：磷脂酸水平、45Ca2 摄取和 [3H]5-羟色胺分泌之间的相关性。
DOI：
10.1016/0003-9861(84)90562-9
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发表时间：
1984
影响因子：
3.9
通讯作者：
Hanahan,DJ
中科院分区：
文献类型：
--
作者：
Shukla,SD;Hanahan,DJ
当32 P标记的兔血小板与5 × 10− 10 m1-O-烷基-2-乙酰基-sn-甘油基-3-磷酸胆碱（AGEPC）一起孵育时，无论是否存在（0.1 mmEGTA）添加的Ca 2+，[32 P]磷脂酸（PA）池在15至20 s内增加了3至5倍。随后[32 P]PA水平在5 min内逐渐降低至接近基础水平。AGEPC以特征性剂量和时间依赖性方式影响[32 P]PA的这种变化。AGEPC的极性头基类似物，如AGEDME和AGEMME，也影响PA标记水平的增加。与以前报道的对兔血小板的活性相当[K.佐藤内河N.平卡德湖M.，McManus和D. J. Hanahan（1981）J.Biol.Chem.256，4425-4432]。其他类似物，即，对兔血小板无活性的lysoGEPC和对映体sn-1-AGEPC对[32 P]PA水平也无影响。兔血小板中PA水平可以通过加入AGEPC而不加入任何Ca ~（2+）来操纵，这一发现为建立Ca ~（2+）摄取、5-羟色胺释放和PA水平之间的相关性提供了一个很好的模型系统。因此，PA必须被视为一个敏感的指标的反应机制，重要的血小板对AGEPC的反应，并可能是在刺激过程中促进钙的吸收的焦点。
When32Pi-labeled rabbit platelets were incubated with 5 × 10−10m1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (AGEPC), either in the presence or absence (0.1 mmEGTA) of added Ca2+, there was a three- to five-fold increase in the [32P]phosphatidic acid (PA) pool within 15 to 20 s. This event was followed by a gradual decrease in the [32P]PA level to near basal level in 5 min. AGEPC effected this change in [32P]PA in a characteristic dose- and time-dependent manner. Polar head group analogs of AGEPC, such as AGEDME and AGEMME, also effected an increase in PA labeling at levels. comparable to those previously reported for their activity toward rabbit platelets [K. Satouchi, R. N. Pinckard, L. M., McManus, and D. J. Hanahan (1981)J. Biol. Chem.256, 4425–4432]. Other analogs, i.e., lysoGEPC and the enantiomer,sn-1-AGEPC, which are inactive toward rabbit platelets, also showed no effect on the level of [32P]PA. The finding that the PA level in rabbit platelets could be manipulated by the addition of AGEPC, without any added Ca2+, provided an excellent model system for establishinig a correlation between the uptake of Ca2+, serotonin release, and PA level. Thus, PA must be regarded as a sensitive indicator of a reaction mechanism important to the platelet response to AGEPC, and could be the focal point in promoting calcium uptake during the stimulation process.