Development of a Sensitive Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for Vitellogenin in the Japanese Eel Anguilla japonica
Development of a Sensitive Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for Vitellogenin in the Japanese Eel Anguilla japonica
复制标题
开发日本鳗鲡中卵黄蛋白原的灵敏夹心酶联免疫吸附测定 (ELISA)
DOI：
10.2331/fishsci.61.283
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发表时间：
1995
影响因子：
1.9
通讯作者：
K. Yamauchi
中科院分区：
文献类型：
--
作者：
Hiromi Okumura;A. Hara;F. Saeki;T. Todo;S. Adachi;K. Yamauchi
利用抗卵黄脂磷蛋白（α-lipovitellin）的特异性抗血清和生物素化的α-lipovitellin的F（ab·ab）2片段，用夹心法进行亲和素-生物素相互作用。该测定可在2天内运行，并常规检测低至0.8 ng/ml的浓度的VTG。在未成熟的鱼中单次注射雌二醇-17 β 1后9-12 h，低水平血清VTG的出现显著增加。建立了VTG ELISA检测方法，可用于血清VTG的定量检测，从而在体外分析卵黄发生的发生和发展的机理。
petitive avidin-biotm interaction was performed by a sandwich method using the specificity of the specific antiserum to eel yolk protein (a-lipovitellin) and biotinylated F(ab•Œ)2 fragment of a-lipovitellin. The assay could be run in 2 days and routinely detect VTG in a concentration as low as 0.8 ng/ml. The appearance of low levels of serum VTG significantly increased 9-12 h after a single injection of es tradiol-17 ƒÀ into immature fish. The development of an ELISA for VTG made possible the quantifica tion of serum VTG and thereby in vitro analysis of the mechanism of the onset and course of vitellogen esis.