c-Yes enhances tumor migration and invasion via PI3K/AKT pathway in epithelial ovarian cancer
c-Yes enhances tumor migration and invasion via PI3K/AKT pathway in epithelial ovarian cancer
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DOI：
10.1016/j.yexmp.2016.06.002
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发表时间：
2016-08-01
影响因子：
3.6
通讯作者：
Xi, Qinghua
中科院分区：
文献类型：
--
作者：
Jin, Yunfeng;Huang, Menghui;Xi, Qinghua
C-Yes的过度表达与几种人类癌症有关。然而，c-Yes在上皮性卵巢癌(EOC)发生中的作用尚不清楚。本研究旨在探讨c-Yes在卵巢癌增殖、转移和侵袭中的作用及相关机制。对119例上皮性卵巢癌标本进行免疫组织化学分析，并与临床病理特征进行对照分析。此外，还进行了EOC样本和细胞系中c-Yes的蛋白质印迹分析，以评估它们的蛋白水平和分子相互作用。Kaplan-Meier生存分析显示，c-Yes的高表达与卵巢上皮性癌预后不良密切相关(P<；0.01)。同时，我们发现c-yes被shRNA敲除后，通过PI3K/AKT途径抑制了EOC细胞的迁移和侵袭能力。综上所述，这些结果表明c-yes在EOC的迁移和入侵中起着重要作用。(C)爱思唯尔公司出版的2016年。
Overexpression of c-Yes has been noted to correlation with several human cancers. However, the effects of c-Yes on epithelial ovarian cancer (EOC) development remain unclear. The aim of this study is going to prove the effects of c-Yes and related mechanisms in proliferation, metastasis and invasion of EOC. Immunohistochemical analysis was performed in 119 human EOC samples, and the data was correlated with clinic pathologic features. Furthermore, western blot analysis is performed for c-Yes in EOC samples and cell lines to evaluate their protein levels and molecular interaction. Kaplan-Meier survival analysis shows that the strong expression of c-Yes exhibited a significant correlation with poor prognosis in human EOC (P < 0.01*). Meanwhile, we found that knockdown of c-Yes by shRNA inhibited the ability of migration and invasion in EOC cells via the PI3K/AKT pathway. In a word, these results suggested that c-Yes plays an important role in migration and invasion of EOC. (C) 2016 Published by Elsevier Inc.