Inhibition of Inducible Liver Enzymes by Endotoxin and Actinomycin D
Inhibition of Inducible Liver Enzymes by Endotoxin and Actinomycin D
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DOI：
10.1128/jb.92.1.107-115.1966
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发表时间：
1966-07
影响因子：
3.2
通讯作者：
L. Berry;D. S. Smythe;L. S. Colwell
中科院分区：
文献类型：
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作者：
L. Berry;D. S. Smythe;L. S. Colwell
贝里湖，加-地乔（宾夕法尼亚州布林莫尔学院），多萝西·S Smythe，and Louise S.科尔韦尔。内毒素和放线菌素D对诱导型肝酶的抑制作用。J. Bacteriol. 92：107-115。1966.- ld 50水平的细菌内毒素降低小鼠肝脏色氨酸吡咯酶，通过同时注射可的松可防止酶诱导16至20小时，显著降低但不防止底物诱导，并且在激素诱导过程中给药时可迅速显著降低酶活性。ld 50量的放线菌素D对色氨酸吡咯酶有类似的作用，除了其对可的松诱导的抑制持续较长的时间。内毒素在完整小鼠中诱导酪氨酸-α-酮戊二酸转氨酶几乎与可的松一样，但在肾上腺切除动物中则不然，这一事实表明该酶的诱导是由于内源性肾上腺激素的释放。另一方面，放线菌素D对这种转氨酶的作用类似于对色氨酸吡咯酶的作用。内毒素和放线菌素D的作用位点对于所研究的两种酶之一似乎是相似的，而对于另一种酶则是不同的，这种关系需要一种特异性，对于内毒素这样复杂的物质来说，这种特异性是难以想象的。
Berry, L. Joe (Bryn Mawr College, Bryn Mawr, Pa.), Dorothy S. Smythe, and Louise S. Colwell. Inhibition of inducible liver enzymes by endotoxin and actinomycin D. J. Bacteriol. 92:107–115. 1966.—Bacterial endotoxin at the ld50 level lowers liver tryptophan pyrrolase in mice, it prevents for 16 to 20 hr the induction of the enzyme by a concurrent injection of cortisone, it lowers significantly but does not prevent substrate induction, and it reduces the enzymatic activity promptly and significantly when administered during the course of hormonal induction. The ld50 amount of actinomycin D has a similar effect on tryptophan pyrrolase, except that its inhibition of induction by cortisone persists for a longer period of time. Endotoxin in the intact mouse induces tyrosine-α-ketoglutarate transaminase almost as well as cortisone, but not in the adrenalectomized animal, a fact that suggests induction of this enzyme is due to release of endogenous adrenal hormones. Actinomycin D, on the other hand, has an effect on this transaminase similar to that on tryptophan pyrrolase. The site of action of endotoxin and actinomycin D would appear to be similar for one of the two enzymes studied and different for the other, a relationship that requires a specificity difficult to imagine for a material as complex as endotoxin.