SV40-INDUCED EPENDYMOMAS IN NEWBORN HAMSTERS .1. VIRUS-TUMOR RELATIONSHIPS
SV40-INDUCED EPENDYMOMAS IN NEWBORN HAMSTERS .1. VIRUS-TUMOR RELATIONSHIPS
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DOI：
10.1016/0042-6822(62)90061-2
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发表时间：
1962-01-01
期刊：
影响因子：
3.7
通讯作者：
KIRSCHSTEIN, RL
中科院分区：
文献类型：
--
作者：
GERBER, P;KIRSCHSTEIN, RL
新生仓鼠脑内接种SV40可形成室管膜瘤。肿瘤发生的潜伏期和频率取决于所使用的病毒浓度。在7个月的观察期内，注射10STCID50或以下病毒的12只动物中的每一只都出现了特征性的肿瘤，而106TCID50或更少的病毒则呈阴性结果。潜伏期87~137天。没有在仓鼠的大脑或其他组织中发现病毒繁殖的证据。证明肿瘤提取液中含有病毒的尝试始终失败。然而，当完整的肿瘤细胞接种在非洲绿猴肾细胞的单层培养上时，发现微量的SV40。只有一小部分肿瘤细胞含有感染性物质。在组织培养的肿瘤细胞中未检测到游离病毒。在7个月的观察中，出生当天接种至少105TCID50/ml的SV1、SV2、SV4或SV5的仓鼠没有表现出任何可检测到的效果。
Intracerebral inoculation of newborn hamsters with SV40 resulted in the formation of ependymomas. The latent period and frequency of tumor incidence depended on the virus concentration employed. Each of 12 animals injected with 10STCID5O of virus developed characteristic tumors, whereas 106TCID50 or less gave negative results during a 7 months'' period of observation. The latent period ranged from 87 to 137 days. No evidence of viral multiplication in the brain or other tissues of the hamster was found. Attempts to demonstrate virus in tumor extracts failed consistently. However, SV40 was found in trace amounts when intact tumor cells were seeded on monolayer cultures of African green monkey kidney cells. Only a small fraction of the tumor cells contained infectious material. No free virus was detectable in tissue-cultured tumor cells. None of the hamsters inoculated with a minimum of 105TCID50/ml of SV1, SV2, SV4, or SV5 on the day of birth showed any detectable effects during 7 months of observation.