In vitro evaluation of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds for bone tissue engineering
In vitro evaluation of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds for bone tissue engineering
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DOI：
10.1016/j.biomaterials.2006.05.025
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发表时间：
2006-10-01
期刊：
影响因子：
14
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
--
作者：
Jiang, Tao;Abdel-Fattah, Wafa I.;Laurencin, Cato T.
三维（3-D）支架是许多组织工程方法中的主要组分之一。通过烧结壳聚糖/聚乳酸-羟基乙酸（PLAGA）复合微球，制备了新型三维壳聚糖/PLAGA复合多孔支架，用于骨组织工程。可以通过控制制造参数（包括烧结温度和烧结时间）来操纵支架的孔径、孔体积和机械性能。烧结的微球支架的总孔体积在28%和37%之间，中值孔径在170-200 μ m的范围内。支架的压缩模量和压缩强度在骨小梁的范围内，使它们适合作为承重骨组织工程的支架。此外，与PLAGA支架相比，MC 3 T3-E1成骨样细胞在复合支架上增殖良好。它还表明，微球表面的壳聚糖的存在下，增加了碱性磷酸酶活性的细胞培养的复合支架和上调基因表达的碱性磷酸酶，骨桥蛋白，骨唾液酸蛋白。(c)2006爱思唯尔有限公司保留所有权利。
A three-dimensional (3-D) scaffold is one of the major components in many tissue engineering approaches. We developed novel 3-D chitosan/poly(lactic acid-glycolic acid) (PLAGA) composite porous scaffolds by sintering together composite chitosan/PLAGA microspheres for bone tissue engineering applications. Pore sizes, pore volume, and mechanical properties of the scaffolds can be manipulated by controlling fabrication parameters, including sintering temperature and sintering time. The sintered microsphere scaffolds had a total pore volume between 28% and 37% with median pore size in the range 170-200 mu m. The compressive modulus and compressive strength of the scaffolds are in the range of trabecular bone making them suitable as scaffolds for load-bearing bone tissue engineering. In addition, MC3T3-E1 osteoblast-like cells proliferated well on the composite scaffolds as compared to PLAGA scaffolds. It was also shown that the presence of chitosan on microsphere surfaces increased the alkaline phosphatase activity of the cells cultured on the composite scaffolds and up-regulated gene expression of alkaline phosphatase, osteopontin, and bone sialoprotein. (c) 2006 Elsevier Ltd. All rights reserved.