Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.
Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.
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DOI：
10.1038/s41598-017-00695-7
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发表时间：
2017-04-06
影响因子：
4.6
通讯作者：
Kaminski CF
中科院分区：
文献类型：
--
作者：
Ströhl F;Lin JQ;Laine RF;Wong HH;Urbančič V;Cagnetta R;Holt CE;Kaminski CF
局部mRNA翻译发生在生长的轴突中，使得能够响应信号精确控制蛋白质组。为了定量测量生长锥中蛋白质合成的时空动态，我们进一步开发了单分子翻译成像（SMTI）技术。我们报道Netrin-1在多个非重复位点触发β-肌动蛋白合成的爆发，特别是在外周。在提示应用后20秒内，反应非常迅速。
Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.