Azithromycin and erythromycin susceptibility and macrolide resistance genes in Prevotella from patients with periodontal disease
Azithromycin and erythromycin susceptibility and macrolide resistance genes in Prevotella from patients with periodontal disease
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DOI：
10.1111/odi.13043
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发表时间：
2019-04-01
期刊：
影响因子：
3.8
通讯作者：
Leon, Ruben
中科院分区：
文献类型：
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作者：
Arredondo, Alexandre;Blanc, Vanessa;Leon, Ruben
目的研究口腔普雷沃菌属。从慢性牙周炎患者中分离，以确定其对阿奇霉素和红霉素的敏感性，并筛选其中存在的大环内酯类耐药基因。材料和方法从52例慢性牙周炎患者的龈下样品中获得具有Prevotella样形态的分离物。通过16 S rRNA基因测序，在种属水平上鉴定每个分离株。在100个普雷沃氏菌属中，结果中间普雷沃菌和变黑普雷沃菌的检出率最高，各占33%。两种抗生素的最小抑菌浓度（MIC）范围为0.016/0.032至>256 μ g/ml。阿奇霉素和红霉素的MIC 50值分别为1.5和1 μ g/ml，两种抗生素的MIC 90值均>256 μ g/ml。19%的分离株携带β-内酰胺酶（B），51%携带β-内酰胺酶（F）。在普雷沃氏菌属中，p53（F）是非常普遍的，我们首次描述了p53（B）基因在普雷沃氏菌属中的存在。任何一个基因的存在似乎与阿奇霉素和红霉素的耐药性程度较高有关。
Objectives To study oral Prevotella spp. isolated from patients with chronic periodontitis, to determine their susceptibility to azithromycin and erythromycin and to screen the presence of macrolide resistance genes therein.Material and Methods Isolates with a Prevotella-like morphology were obtained from subgingival samples of 52 patients with chronic periodontitis. Each isolate was identified to the species level by sequencing of the 16S rRNA gene. In 100 Prevotella spp. isolates, azithromycin and erythromycin susceptibility was determined using the E test method, and the screening of erm(A), erm(B), erm(C), erm(F), erm(G), erm(Q) and mef(A) genes was done by PCR.Results Prevotella intermedia and Prevotella nigrescens were the most identified species (33% each). Minimum inhibitory concentrations (MICs) ranges for both antibiotics were 0.016/0.032 to >256 mu g/ml. MIC50 values for azithromycin and erythromycin were 1.5 and 1 mu g/ml, respectively, and MIC90 values were >256 mu g/ml for both antibiotics. Nineteen per cent of the isolates carried erm(B), and 51% carried erm(F).Conclusions The MIC values found were high compared to previous studies. erm(F) was greatly prevalent, and we describe for the first time the erm(B) gene in Prevotella spp. The presence of either of the genes seems to be associated with a higher degree of resistance to azithromycin and erythromycin.