Effects of irbesartan on the growth and differentiation of adipocytes in obese Zucker rats
Effects of irbesartan on the growth and differentiation of adipocytes in obese Zucker rats
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DOI：
10.1038/oby.2005.235
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发表时间：
2005-11-01
期刊：
影响因子：
--
通讯作者：
D'Amico, M
中科院分区：
文献类型：
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作者：
Di Filippo, C;Lampa, E;D'Amico, M
目的：探讨选择性血管紧张素受体I拮抗剂伊贝沙坦对肥胖Zucker FA/FA大鼠脂肪细胞生长和分化的影响。研究方法和步骤：肥胖Zucker FA/FA大鼠口服厄贝沙坦3~10~30 mg/(kg·d)，连续3周。通过分析白色(腹膜后)或棕色(肩胛间)脂肪组织样本中是否存在过氧化体增殖物激活受体γ、瘦素和甘油-3-磷酸脱氢酶活性来评价脂肪细胞的分化。结果：厄贝沙坦治疗肥胖症Zucker/FA可以有效地减少棕色(肩峰间)和白色(腹膜后)脂肪组织中脂肪细胞的分化。事实上，厄贝沙坦显著(p<；0.01)并呈剂量依赖性地降低组织中瘦素、过氧化体增殖物激活受体γ的水平，以及脂肪细胞分化的标志物甘油-3-磷酸脱氢酶的活性。实验剂量的厄贝沙坦均不影响非肥胖大鼠的这些标志物。讨论：血管紧张素受体I受体拮抗厄贝沙坦降低肥胖Zucker大鼠血管紧张素11的成脂活性，终点是减少脂肪组织内脂肪细胞的生长和分化。
Objective: The aim of this study was to evaluate the effects of the selective angiotensin receptor I antagonist irbesartan on the growth and differentiation of the adipocytes in obese Zucker fa/fa rats.Research Methods and Procedures: Obese Zucker fa/fa rats were treated by oral route for 3 weeks with irbesartan at doses of 3-10-30 mg/kg per day. The adipocyte differentiation was evaluated by analyzing tissue samples of white (retroperitoneal) or brown (interscapular) adipose tissue for the presence of peroxisome proliferator activated receptor gamma, leptin, and the activity of glycerol-3-phosphate dehydrogenase.Results: This study showed that the treatment of obese Zucker fa/fa with irbesartan effectively reduced the differentiation of adipocytes within brown (interscapular) and white (retroperitoneal) adipose tissue. In fact, irbesartan significantly (p < 0.01) and dose-dependently reduced the tissue levels of leptin, peroxisome proliferator activated receptor gamma, and the activity of the enzyme glycerol-3-phoshate dehydrogenase accepted markers of adipocyte differentiation. None of the tested doses of irbesartan affected these markers in non-obese rats.Discussion: The antagonism of the angiotensin receptor I receptors with irbesartan reduces the adipogenic activity of angiotensin 11 in obese Zucker rats, with the endpoint being reduction of the growth and differentiation of the adipocytes within the adipose tissue.