Analysis of telomeres and telomerase.
Analysis of telomeres and telomerase.
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DOI：
10.1002/0471143030.cb1806s20
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发表时间：
2003-11-01
影响因子：
--
通讯作者：
Wright, Woodring E
中科院分区：
文献类型：
--
作者：
Herbert, Brittney-Shea;Shay, Jerry W;Wright, Woodring E
该单元描述了分析人类细胞端粒长度和端粒酶活性的技术。端粒长度可以通过改良的Southern印迹法测定，其中染色体末端限制性片段（TRF）的分析提供细胞群体中所有端粒的平均长度。端粒酶活性可以通过基于聚合酶链反应（PCR）的灵敏且有效的检测方法（也称为端粒重复扩增方案（TRAP））在体外测量。这些测定可用于研究衰老和癌症治疗对端粒生物学和端粒酶活性的体外细胞效应。
This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also known as telomeric repeat amplification protocol (TRAP). These assays can be used to study the in vitro cellular effects of aging and cancer treatments on telomere biology and telomerase activity.