Hydrogen gas promotes the adventitious rooting in cucumber under cadmium stress
Hydrogen gas promotes the adventitious rooting in cucumber under cadmium stress
复制标题
镉胁迫下氢气促进黄瓜不定根
DOI：
10.1371/journal.pone.0212639
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发表时间：
2019-02-20
期刊：
影响因子：
3.7
通讯作者：
Liao, Weibiao
中科院分区：
文献类型：
--
作者：
Wang, Bo;Bian, Biting;Liao, Weibiao
氢气（H2）在植物发育和逆境响应中起着重要作用。以黄瓜（Cucumis sativus L.）外植体为材料，研究了镉胁迫下H2在黄瓜不定根发育中的作用及其生理机制。结果表明：富氢水（HRW）促进了Cd胁迫下的不定根，其中50%的富氢水获得了最大的生物响应。与Cd处理相比，HRW + Cd处理显著降低了丙二醛（MDA）、过氧化氢（H2O2）、超氧自由基（O2-）、硫代巴比妥酸活性物质（TBARS）、抗坏血酸（AsA）和还原性谷胱甘肽（GSH）含量，显著降低了相对电导率（REC）、脂氧合酶（LOX）活性、AsA/二十二碳六烯酸（DHA）比值和GSH/氧化谷胱甘肽（GSSG）比值，显著提高了DHA和GSSG含量。HRW + Cd处理也显著提高了抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、单脱氢抗坏血酸还原酶（MDHAR）和谷胱甘肽还原酶（GR）的活性和相关基因表达。HRW + Cd处理显著降低了吲哚乙酸氧化酶（IAAO）活性，提高了渗透调节物质含量，提高了过氧化物酶（POD）和多酚氧化酶（PPO）活性。综上所述，在Cd胁迫下，H2可以通过降低氧化损伤、增加渗透调节物质含量和调节生根相关酶活性来诱导不定根。
Hydrogen gas (H2) plays an important role in plant development and stress responses. Here, cucumber (Cucumis sativus L.) explants were used to investigate the roles of H2 in adventitious root development under cadmium (Cd) stress and its physiological mechanism. The results showed that hydrogen-rich water (HRW) promoted adventitious rooting under Cd stress and 50% HRW obtained the maximal biological response. Compared with Cd treatment, HRW + Cd treatment significantly reduced the content of malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide radical (O2-), thiobarbituric acid reactive substances (TBARS), ascorbic acid (AsA) and reduced glutathione (GSH), as well as relative electrical conductivity (REC), lipoxygenase (LOX) activity, AsA/docosahexaenoic acid (DHA) ratio, and GSH/oxidized glutathione (GSSG) ratio, while increasing DHA and GSSG content. HRW + Cd treatment also significantly increased in the activity and related gene expression of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR). Additionally, HRW + Cd treatment increased the contents of osmotic adjustment substances, as well as the activities of peroxidase (POD) and polyphenol oxidase (PPO), while significantly decreasing indoleacetic acid oxidase (IAAO) activity. In summary, H2 could induce adventitious rooting under Cd stress by decreasing the oxidative damage, increasing osmotic adjustment substance content and regulating rooting-related enzyme activity.