Quantitative differences in lipid raft components between murine CD4+ and CD8+ T cells.
Quantitative differences in lipid raft components between murine CD4+ and CD8+ T cells.
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DOI：
10.1186/1471-2172-5-2
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发表时间：
2004-01-30
期刊：
影响因子：
3
通讯作者：
Taub DD
中科院分区：
文献类型：
--
作者：
de Mello Coelho V;Nguyen D;Giri B;Bunbury A;Schaffer E;Taub DD
脂筏已被证明在T细胞成熟、活化以及在CD 4+辅助和CD 8+细胞毒性T细胞中免疫突触的形成中发挥作用。然而，CD 4+和CD 8 + T细胞之间的脂筏组分的差异表达仍然不清楚。为了研究这个问题，我们分析了年轻和老年小鼠T细胞中GM 1的表达，以及小鼠CD 4+和CD 8 + T细胞亚群中糖基磷脂酰肌醇（GPI）连接蛋白Thy-1和胆固醇的表达。我们发现，CD 4 + CD 8-和CD 8 + CD 4-胸腺细胞在不同的成熟阶段显示不同的GM 1表面表达。这种现象并没有随着逐渐衰老而改变，因为这些发现在小鼠的整个寿命期间是一致的。在外周中，CD 8 + T细胞比CD 4 + T细胞表达显著更高水平的GM 1。此外，我们观察到GM 1水平在CD 8 + T细胞上随着年龄的增长而增加，但在CD 4 + T细胞中则没有。我们还证实了幼稚（CD 44 lo）和记忆（CD 44 hi）CD 8 + T细胞以及幼稚和记忆CD 4 + T细胞在其表面上表达相似水平的GM 1。此外，我们发现，与CD 4 + T细胞相比，CD 8 + T细胞表达更高水平的与脂筏结构域相关的GPI锚定的细胞表面蛋白Thy-1。最后，我们观察到CD 8 + T细胞中的总细胞胆固醇水平高于CD 4 + T细胞。这些结果表明，在年轻和老年小鼠的CD 4+和CD 8 + T细胞之间的脂筏组分的异质性。脂筏组成的这种差异可能有助于差异的CD 4和CD 8分子信号传导途径，以及可能的效应器反应介导的这些T细胞亚群后TCR活化。
Lipid rafts have been shown to play a role in T cell maturation, activation as well as in the formation of immunological synapses in CD4+ helper and CD8+ cytotoxic T cells. However, the differential expression of lipid raft components between CD4+ and CD8+ T cells is still poorly defined. To examine this question, we analyzed the expression of GM1 in T cells from young and aged mice as well as the expression of the glycosylphosphatidylinositol (GPI)-linked protein Thy-1 and cholesterol in murine CD4+ and CD8+ T cell subpopulations. We found that CD4+CD8- and CD8+CD4- thymocytes at different stages of maturation display distinct GM1 surface expression. This phenomenon did not change with progressive aging, as these findings were consistent over the lifespan of the mouse. In the periphery, CD8+ T cells express significantly higher levels of GM1 than CD4+ T cells. In addition, we observed that GM1 levels increase over aging on CD8+ T cells but not in CD4+ T cells. We also verified that naïve (CD44lo) and memory (CD44hi) CD8+ T cells as well as naïve and memory CD4+ T cells express similar levels of GM1 on their surface. Furthermore, we found that CD8+ T cells express higher levels of the GPI-anchored cell surface protein Thy-1 associated with lipid raft domains as compared to CD4+ T cells. Finally, we observed higher levels of total cellular cholesterol in CD8+ T cells than CD4+ T cells. These results demonstrate heterogeneity of lipid raft components between CD4+ and CD8+ T cells in young and aged mice. Such differences in lipid raft composition may contribute to the differential CD4 and CD8 molecule signaling pathways as well as possibly to the effector responses mediated by these T cell subsets following TCR activation.