A genome-wide identification and analysis of the homeobox genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
A genome-wide identification and analysis of the homeobox genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
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褐飞虱 Nilaparvata lugens（半翅目：飞虱科）同源盒基因的全基因组鉴定和分析
DOI：
10.1002/arch.21833
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发表时间：
2021-07-19
影响因子：
2.2
通讯作者：
Xu，Hai-Jun
中科院分区：
文献类型：
--
作者：
Fu，Sheng-Jie;Zhang，Jin-Li;Xu，Hai-Jun
同源异型盒家族是一个庞大而多样的基因超类，其中许多基因作为转录因子在动物组织分化和胚胎发生中发挥重要作用。褐飞虱（Nilaparvata lugens）是亚洲最具破坏性的水稻害虫，其高繁殖力是其在自然和农业生态中取得成功的重要原因。本研究共鉴定出94个BPH同源异型盒基因，可分为9类75个基因家族。这个数字与蜜蜂中发现的同源盒基因的数量相当，但略低于果蝇或赤拟谷盗。时空分析表明，大多数BPH同源盒基因以发育和组织特异性方式表达，其中21个基因在卵巢中高表达。RNA干扰（RNAi）介导的功能分析表明，22个同源盒基因在若虫发育和若虫向成虫过渡过程中起重要作用，而67个基因在此过程中被阻断。生殖力测定结果表明，13个卵巢偏向基因（zfh1，schlank，abd‐ A，Lim3_2，Lmxb，Prop，ap_1，Not，lab，Hmx，vis，Pknox，C15）的敲低导致了生殖缺陷。这是对半翅目昆虫同源盒基因的首次全面研究，有助于我们理解同源盒基因在昆虫生殖中的功能意义。
The homeobox family is a large and diverse superclass of genes, many of which act as transcription factors that play important roles in tissue differentiation and embryogenesis in animals. The brown planthopper (BPH),Nilaparvata lugens, is the most destructive pest of rice in Asia, and high fecundity contributes significantly to its ecological success in natural and agricultural habits. Here, we identified 94 homeobox genes in BPH, which could be divided into 75 gene families and 9 classes. This number is comparable to the number of homeobox genes found in the honeybeeApis mellifera, but is slightly less than inDrosophilaor the red flour beetleTribolium castaneum. A spatio‐temporal analysis indicated that most BPH homeobox genes were expressed in a development and tissue‐specific manner, of which 21 genes were highly expressed in ovaries. RNA interference (RNAi)‐mediated functional assay showed that 22 homeobox genes were important for nymph development and the nymph to adult transition, whereas 67 genes were dispensable during this process. Fecundity assay showed that knockdown of 13 ovary‐biased genes (zfh1,schlank,abd‐A,Lim3_2,Lmxb,Prop,ap_1,Not,lab,Hmx,vis,Pknox, andC15) led to the reproductive defect. This is the first comprehensive investigation into homeobox genes in a hemipteran insect and thus helps us to understand the functional significance of homeobox genes in insect reproduction.