CHARACTERIZATION OF THE 5'-END REGION AND THE 1ST 2 EXONS OF THE BETA-PROTEIN PRECURSOR GENE
CHARACTERIZATION OF THE 5'-END REGION AND THE 1ST 2 EXONS OF THE BETA-PROTEIN PRECURSOR GENE
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DOI：
10.1016/0006-291x(89)92437-6
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发表时间：
1989-02-28
影响因子：
3.1
通讯作者：
ROBAKIS, NK
中科院分区：
文献类型：
--
作者：
LAFAUCI, G;LAHIRI, DK;ROBAKIS, NK
分离到了编码β-淀粉样蛋白前体（β-APP）基因启动子区和前两个外显子的人类基因组克隆。第一个外显子长205个碱基对，编码19个氨基酸。第二个外显子长168 bp，编码56个氨基酸。β-APP基因的5 '侧序列在包括PC12细胞在内的几种细胞系中显示出最高活性的启动子活性。该基因的启动子区域缺乏通常与真核生物启动子相关的典型“TATAA”和“CAAT”盒子。ggggcgc序列的5个拷贝位于- 107和- 188之间，一个拷贝位于β-APP基因的第一个外显子内。转录因子Sp1和AP-1识别的共识序列位于RNA起始位点的上游。在主要的转录起始位点周围发现了能够形成稳定的发夹状结构的回文序列。β-APP启动子的结构特征表明，该基因的表达有多种因素参与调控。
Human genomic clones encoding the promoter region and the first two exons of the β-amyloid protein precursor (β-APP) gene were isolated. The first exon is 205 base pairs (bp) long and encodes 19 amino acids. The second exon is 168 bp long and encodes 56 amino acids. The 5′-flanking sequence of the β-APP gene was found to display promoter activity in several cell lines including PC12 cells where the highest activity was detected. The promoter region of this gene lacks the typical “TATAA” and “CAAT” boxes usually associated with eukaryotic promoters. Five copies of the GGGCGC sequence are located between positions −107 and −188 and one copy is located within the first exon of the β-APP gene. Consensus sequences recognized by the transcription factors Sp1 and AP-1 are located upstream from the RNA start site. Palindromic sequences capable of forming stable hairpin-like structures are found aroud the main transcription initiation site. The structural characteristics of the β-APP promoter indicate that multiple elements participate in the regulation of the expression of this gene.