Lipopolysaccharide in ovarian follicular fluid influences the steroid production in large follicles of dairy cows
Lipopolysaccharide in ovarian follicular fluid influences the steroid production in large follicles of dairy cows
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DOI：
10.1016/j.anireprosci.2013.11.005
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发表时间：
2014-01-10
影响因子：
2.2
通讯作者：
Shimizu, Takashi
中科院分区：
文献类型：
--
作者：
Magata, Fumie;Horiuchi, Maya;Shimizu, Takashi
在产后奶牛中，各种炎症性疾病会降低繁殖性能。来自子宫或乳腺感染革兰氏阴性菌的脂多糖（LPS）被证明可以抑制体外卵泡颗粒细胞中类固醇的产生。本研究旨在探讨卵巢卵泡液中脂多糖与体内大卵泡的卵泡膜和颗粒细胞生成甾体的关系。从屠宰场采集牛卵巢，使用最大（F1）和第二大（F2）卵泡（直径约8mm， n=38）。采用定量动力学法测定卵泡液中LPS浓度。通过测定卵泡液中雌二醇（E2）和孕酮（P4）浓度以及分析卵泡和颗粒细胞中类固醇生成相关基因的转录水平来评估卵泡类固醇生成。卵泡液中LPS浓度为0.2 ~ 2.0 EU/mL。高脂多糖水平卵泡（>0.5 EU/mL, n = 15）中E2浓度较低，P4浓度较高(
In postpartum dairy cows, various inflammatory diseases depress reproductive performance. Lipopolysaccharide (LPS) derived from infections of the uterus or mammary gland with Gram-negative bacteria was shown to suppress steroid production in the granulosa cells of follicles in vitro. The aim of the study was to investigate the relationship between LPS in ovarian follicular fluid and steroidogenesis by the theca and granulosa cells of the large follicles in vivo. Bovine ovaries were collected from a slaughterhouse, and the largest (F1) and the second largest (F2) follicles were used (>8 mm in diameter, n=38). LPS concentration in the follicular fluid was measured using quantitative kinetic assay. Follicular steroidogenesis was evaluated by measuring the estradiol (E2) and progesterone (P4) concentration in follicular fluid and by analysing transcription levels of steroidogenesis-related genes in theca and granulosa cells. LPS concentration detected in follicular fluid ranged from 0.2 to 2.0 EU/mL. In follicles with a high level of LPS (>0.5 EU/mL, n = 15), the concentration of E2 was lower and that of P4 was higher when compared to those in follicles with a low level of LPS (