PLATELET-DERIVED GROWTH-FACTOR STIMULATES SYNTHESIS OF 1,2-DIACYLGLYCEROL FROM MONOACYLGLYCEROL IN BALB/C 3T3-CELLS
PLATELET-DERIVED GROWTH-FACTOR STIMULATES SYNTHESIS OF 1,2-DIACYLGLYCEROL FROM MONOACYLGLYCEROL IN BALB/C 3T3-CELLS
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DOI：
10.1042/bj2620947
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发表时间：
1989-09-15
影响因子：
4.1
通讯作者：
KOJIMA, I
中科院分区：
文献类型：
--
作者：
HATA, Y;OGATA, E;KOJIMA, I
1,2-Diacylglycerol (1,2-DAG) plays an important role in the protein kinase C-mediated signal-transduction system. Several reports have shown that 1,2-DAG is generated through various pathways other then classical phospholipid hydrolysis. We observed a rapid incorporation of [3H]myristate into 1,2-DAG in platelet-derived-growth-factor (PDGF)-treated Balb/c 3T3 cells. [14C]Palmitate was similarly incorporated into 1,2-DAG. The effect of PDGF, which was inhibited by cycloheximide, became maximal after 60 min treatment with PDGF, and disappeared 300 min after removal of PDGF. Treatment with triacylglycerol lipase revealed that labelled saturated fatty acid was incorporated into the sn-1 positiion. PDGF barely stimulated incorporation of [3H]glycerol or [14C]glucose into 1,2-DAG. Incorporation of [3H]myristate into 1,2-DAG preceded that into triacyglycerol and phospholipids. These results suggest that synthesis of 1,2-DAG from monoacylglycerol is enhanced in PDGF-treated cells. However, there is no significant difference in the activity of monoacylglycerol acyltransferase measured in vitro in quiescent and PDGF-treated cells. The reason for this discrepancy is disussed.
