The activity of powdery-mildew haustoria after feeding the host cells with different sugars, as measured with a potentiometric cyanine dye
The activity of powdery-mildew haustoria after feeding the host cells with different sugars, as measured with a potentiometric cyanine dye
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DOI：
10.1007/bf00394782
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发表时间：
1988-05
期刊：
影响因子：
4.3
通讯作者：
K. Mendgen;P. Nass
中科院分区：
文献类型：
--
作者：
K. Mendgen;P. Nass
小麦白粉病菌Erysiphe graminis f.大麦属植物在其寄主大麦的细胞内产生吸器。为了确定这些吸器的生理活性，研究了吸器线粒体中跨膜的电位。膜电位用荧光电位计花青染料碘化3，3 ′-二丁基氧碳菁测定。向感染的细胞中加入去极化剂（羰基氰-氯苯腙、2，4-二硝基苯酚或KCN）导致在加入低浓度后荧光增加或在加入较高浓度后荧光减少。当被感染的宿主细胞被喂以增加浓度的d-葡萄糖（25，50，75 mM），相应的荧光的减少立即在真菌吸器的线粒体中测量。蔗糖在20分钟后引起类似的荧光减弱，d-半乳糖和d-果糖引起的荧光减弱稍小，l-葡萄糖和d-葡萄糖醇没有影响。结果表明，吸器能迅速吸收宿主细胞中的葡萄糖。蔗糖、d-半乳糖和d-果糖在其产物到达真菌吸器或线粒体之前似乎需要时间代谢。
The biotrophic parasiteErysiphe graminis f. sp. hordeiproduces haustoria within the cells of its hostHordeum vulgare. To determine the physiological activity of these haustoria, the electric potential across the membranes in the mitochondria of the haustorium was studied. The membrane potential was estimated with the fluorescent potentiometric cyanine dye 3,3′-dibutyloxacarbocyanine iodide. The addition of depolarizing agents (carbonylcyanidem-chlorophenylhydrazone, 2,4-dinitrophenol or KCN) to infected cells resulted in an increase of fluorescence after the addition of low concentrations or a decrease of fluorescence after the addition of higher concentrations. When the infected host cell was fed with increasing concentrations ofd-glucose (25, 50, 75 mM), corresponding decreases of fluorescence were measured immediately in the mitochondria of the fungal haustoria. Sucrose induced a similar reduction of fluorescence about 20 min late.d-Galactose andd-fructose induced a somewhat smaller reduction of fluorescence,l-glucose andd-glucitol had no effect. The results indicate that haustoria take up glucose from the host cells immediately. Sucrose,d-galactose andd-fructose seem to require time to be metabolized before their products reach the fungal haustorium or mitochondria.