Leucine turnover and oxidation in trained rats during exercise.
Leucine turnover and oxidation in trained rats during exercise.
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受过训练的大鼠运动期间的亮氨酸周转和氧化。
DOI：
10.1152/ajpendo.1985.249.2.e137
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Brooks,GA
中科院分区：
文献类型：
--
作者：
Henderson,SA;Black,AL;Brooks,GA
研究了运动训练对幼龄吸收后雌性大鼠安静和运动过程中亮氨酸代谢和氧化的影响。通过使用[1-14C]亮氨酸的持续输注方法，初步研究了两者之间的关系。测定动脉血中亮氨酸的比活度，监测呼出空气中O2的消耗、二氧化碳的产生和14CO2的比活度。训练大鼠的亮氨酸周转率比未训练的大鼠高70%，且不受运动的影响。由于更高的周转率，在训练的大鼠中，无论是在休息还是运动中，亮氨酸氧化的速度都要高出40%。此外，亮氨酸氧化与代谢率成正比，占安静时亮氨酸周转率的30%，占运动时亮氨酸周转率的40%~60%。在同一动物中，通过测量输注4wk后血红蛋白池中[1-14C]亮氨酸剂量的比例(占位示踪法)来二次评估亮氨酸转换。亮氨酸周转率的占有值与持续输液法测定的值没有差别。结果表明，训练增加了全身亮氨酸的周转率，训练和运动均增加了亮氨酸的氧化。
The effects of training on leucine turnover and oxidation were studied during rest and exercise in young, postabsorptive female rats. Relationships were primarily studied by the continuous infusion method, using [1-14C]leucine. Arterial blood was assayed for leucine specific activity and expired air was monitored for O2 consumption, CO2 production, and the specific activity of 14CO2. The rate of leucine turnover in trained rats was 70% greater than the rate of leucine turnover in untrained rats and was not affected by exercise. By virtue of a higher turnover, the rate of leucine oxidation was 40% greater in trained rats during both rest and exercise. Moreover, leucine oxidation was proportional to the metabolic rate and represented 30% of the leucine turnover at rest and 40-60% of the leucine turnover during exercise. In the same animals leucine turnover was secondarily assessed by measuring the fraction of the [1-14C]leucine dose contained in the hemoglobin pool 4 wk after infusion (occupancy tracer method). Occupancy values for leucine turnover were not different from those determined by the continuous infusion method. The results show that whole body leucine turnover is increased by training and that leucine oxidation is increased by both training and exercise.