DiversiWcation of three APETALA1/FRUITFULL-like genes in wheat
DiversiWcation of three APETALA1/FRUITFULL-like genes in wheat
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DOI：
10.1007/s00438-012-0679-7
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发表时间：
2012-04-01
影响因子：
3.1
通讯作者：
Murai, Koji
中科院分区：
文献类型：
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作者：
Kinjo, Hiroko;Shitsukawa, Naoki;Murai, Koji
禾本科物种的基因组具有APETALA 1/FRUITFULL（AP 1/FUL）样基因的三个旁系同源物（FUL 1、FUL 2和FUL 3），其源自FUL谱系。在本研究中，我们重点研究了小麦AP 1/FUL-like基因WFUL 1（与VRN 1相同），WFUL 2和WFUL 3在营养生长向生殖生长过渡过程中的不同作用。序列分析表明，有一个高水平的变异性的C-结构域的氨基酸序列之间的WFUL基因。利用春小麦品种中国春的表达分析表明，WFUL 1/VRN 1表达在叶片以及穗原基的非春化植物在营养阶段之前的相位转换，而WFUL 2和WFUL 3在叶片中不表达。这一结果表明，WFUL 1/VRN 1执行一个独特的作用，在叶相变之前。在幼穗中，WFUL 1/VRN 1和WFUL 3在所有发育中的花器官中表达，而WFUL 2的表达仅限于外稃和内稃的花器官。此外，酵母双杂交和三杂交分析表明，WFUL 2，而不是WFUL 1/VRN 1或WFUL 3，与B类和E类蛋白相互作用。这些结果表明，小麦WFUL 2基因与B类基因和E类基因协同作用，具有确定花分生组织和花外器官（外稃和内稃）身份的A类功能。
The genomes of grass family species have three paralogs of APETALA1/FRUITFULL (AP1/FUL)-like genes (FUL1, FUL2 and FUL3) that are derived from the FUL lineage. In this study, we focus on the different roles of the wheat AP1/FUL-like genes, WFUL1 (identical to VRN1), WFUL2 and WFUL3, during the transition from vegetative to reproductive growth. Sequence analysis indicated that there was a high level of variability in the amino acid sequence of the C-domain among three WFUL genes. Expression analyses using the spring wheat cultivar Chinese Spring indicated that WFUL1/VRN1 was expressed in leaves as well as spike primordia of non-vernalized plants at the vegetative stage just before phase transition, while WFUL2 and WFUL3 were not expressed in leaves. This result indicates that WFUL1/VRN1 performs a distinct role in leaves before phase transition. In young spikes, WFUL1/VRN1 and WFUL3 were expressed in all developing floral organs, whereas WFUL2 expression was restricted in the floral organs to the lemma and palea. Furthermore, yeast two-hybrid and three-hybrid analyses revealed that WFUL2, but not WFUL1/VRN1 or WFUL3, interacted with class B and class E proteins. These results suggest that WFUL2 of wheat has class A functions in specifying the identities of floral meristems and outer floral organs (lemma and palea) through collaboration with class B and class E genes.