Preparation of newly identified polysaccharide fromPleurotus eryngiiand its anti-inflammation activities potential
Preparation of newly identified polysaccharide fromPleurotus eryngiiand its anti-inflammation activities potential
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DOI：
10.1111/1750-3841.15375
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发表时间：
2020-08-14
影响因子：
3.9
通讯作者：
Xiao, Hang
中科院分区：
文献类型：
--
作者：
Ma, Gaoxing;Kimatu, Benard Muinde;Xiao, Hang
本研究采用脂多糖（LPS）刺激RAW264.7巨噬细胞，测定了两种新鉴定的杏白菇多糖（WPEP， NPEP）的抗炎作用。表征分析表明，WPEP和NPEP的分子量分别为167和274 kDa，主要由葡萄糖和β型糖苷键组成。WPEP和NPEP可通过调节NO、前列腺素E2 （PGE(2)）、白细胞介素-1 β (IL-1 β)、肿瘤坏死因子- α (tnf - α)和白细胞介素-6 （IL-6）的产生，显著抑制lps诱导的炎症反应。这是通过抑制p38的磷酸化、蛋白激酶1/2的细胞外调节和应激激活蛋白激酶/jun氨基末端激酶来阻断丝裂原活化蛋白激酶（MAPK）途径的激活。此外，WPEP和NPEP通过减少核易位和p65磷酸化抑制NF-kappa B信号传导。总的来说，我们的结果，首次确定了两个op。表明其具有抗炎作用，表明其具有良好的应用潜力。枸杞多糖作为特定的功能性食品。本研究制备并表征了新鉴定的杏鲍菇水溶性多糖组分，并阐明了其营养价值，主要是利用脂多糖（LPS）刺激RAW264.7细胞的抗炎活性相关的免疫应答。综上所述，本研究结果提示p。茯苓多糖是开发新型免疫调节剂或功能性补充食品的潜在候选物质。
The anti-inflammatory effects of two newly identifiedPleurotus eryngiipolysaccharides (WPEP, NPEP) were determined in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in this study. Characterization analysis revealed that molecular weights of WPEP and NPEP were 167 and 274 kDa, and were mainly composed of glucose with beta-type glycosidic linkages. WPEP and NPEP could significantly inhibit LPS-induced inflammatory responses by regulating the production of NO, Protaglandin E2 (PGE(2)), Interleukin-1 beta (IL-1 beta), Tumor necrosis factor-alpha (TNF-alpha), and Interleukin-6 (IL-6). This was through the blocking of the activation of Mitogen-activated protein kinase (MAPK) pathway by inhibiting phosphorylation of p38, extracellular regulation of protein kinases 1/2, and stress-activated protein kinase/jun aminoterminal kinase. Moreover, WPEP and NPEP inhibited NF-kappa B signaling by reducing nuclear translocation and phosphorylation of p65. Overall, our results, for the first time identified twoP. eryngiipolysaccharides and demonstrated the related anti-inflammatory effects, which indicated the favorable potential ofP. eryngiipolysaccharide as specific functional foods. Practical Application This study prepared and characterized newly identifiedPleurotus eryngiiwater-soluble polysaccharide fractions and elucidated the nutritional benefits, mainly the immune response related to anti-inflammatory activities by utilizing lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Collectively, results of this study suggested that theP. eryngiipolysaccharide fractions could be considered as potential candidates for exploration in the development of new immunomodulatory agent or functional supplementary foods.