CLAMP and Zelda function together to promote Drosophila zygotic genome activation.
CLAMP and Zelda function together to promote Drosophila zygotic genome activation.
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DOI：
10.7554/elife.69937
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发表时间：
2021-08-03
期刊：
影响因子：
7.7
通讯作者：
Larschan E
中科院分区：
文献类型：
--
作者：
Duan J;Rieder L;Colonnetta MM;Huang A;Mckenney M;Watters S;Deshpande G;Jordan W;Fawzi N;Larschan E
在合子基因组激活（zygotic genome activation， ZGA）这一重要而保守的过程中，染色质可及性必须增加才能促进转录。果蝇是定义驱动ZGA机制的成熟模型。Zelda （ZLD）是在果蝇胚胎中促进ZGA的关键先锋转录因子（TF）。然而，许多含有富含ga基序的基因组位点在ZGA期间可以独立于ZLD而被访问。因此，我们假设其他与ZLD一起起作用的早期tf尚未被确定，特别是那些能够与富含ga的基序结合的tf，如男性特异性致死蛋白（MSL）的染色质连接适配器（CLAMP）。在这里，我们证明果蝇胚胎发育需要母体CLAMP来(1)激活合子转录；(2)增加通常编码其他必需tf的特定基因启动子的染色质可及性；(3)增强染色质可及性，促进关键胚胎启动子子集的ZLD占用。因此，CLAMP作为一个先锋因子，在ZGA中起着有针对性但又必不可少的作用。
During the essential and conserved process of zygotic genome activation (ZGA), chromatin accessibility must increase to promote transcription. Drosophila is a well-established model for defining mechanisms that drive ZGA. Zelda (ZLD) is a key pioneer transcription factor (TF) that promotes ZGA in the Drosophila embryo. However, many genomic loci that contain GA-rich motifs become accessible during ZGA independent of ZLD. Therefore, we hypothesized that other early TFs that function with ZLD have not yet been identified, especially those that are capable of binding to GA-rich motifs such as chromatin-linked adaptor for male-specific lethal (MSL) proteins (CLAMP). Here, we demonstrate that Drosophila embryonic development requires maternal CLAMP to (1) activate zygotic transcription; (2) increase chromatin accessibility at promoters of specific genes that often encode other essential TFs; and (3) enhance chromatin accessibility and facilitate ZLD occupancy at a subset of key embryonic promoters. Thus, CLAMP functions as a pioneer factor that plays a targeted yet essential role in ZGA.
影响因子：
4.4
作者：
Tariq M;Wegrzyn R;Anwar S;Bukau B;Paro R
通讯作者：
Paro R