Fluviibacter phosphoraccumulans gen. nov., sp. nov., a polyphosphate accumulating bacterium of Fluviibacteraceae fam. nov., isolated from surface river water
Fluviibacter phosphoraccumulans gen. nov., sp. nov., a polyphosphate accumulating bacterium of Fluviibacteraceae fam. nov., isolated from surface river water
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磷积聚河杆菌属
DOI：
10.1099/ijsem.0.004446
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发表时间：
2020
影响因子：
2.8
通讯作者：
Wataru Suda
中科院分区：
文献类型：
--
作者：
Keiji Watanabe;Sho Morohoshi;Tadao Kunihiro;Yuichi Ishii;Lena Takayasu;Yusuke Ogata;Chie Shindo;Wataru Suda
从日本埼玉县地表水中分离到3株需氧、革兰氏染色阴性、无运动的杆状细菌，分别为SHINM1T、ICHIJ1和ICHIAU1。基于16S rRNA和40个标记基因序列的系统发育分析表明，该菌株在红环目中形成了一个独特的系统发育谱系。3株16S rRNA基因具有100%的相似性。在15-30°C和pH 6.0-9.5的条件下生长，但在≥1.0% (w/v) NaCl的存在下不生长。分离株细胞内多磷酸盐颗粒染色阳性。主要细胞脂肪酸为C16: 0、总特征2 （C12: 1醛和/或iso-C16: 1I和/或C14: 03-OH）、总特征3 （C16: 1ω7cand/或C16: 1ω6c）和总特征8 （C18: 1ω7cand/或C18: 1ω6c）。主要极性脂质为磷脂酰乙醇胺和一种未识别的磷脂。菌株shinm1t1的主要醌系为泛素-8，其DNA G+C含量为56.7% mol%。基因组测序结果显示，3株分离菌株的基因组大小为2.29 ~ 2.43 Mbp，同源性平均值≥98.9%。根据表型和系统发育分析结果，菌株SHINM1T， ICHIJ1和ICHIAU1代表了一个新属的新种，其名称为fluviibacter phospaccumulansgen。11月11日被提议在一个新的家族中，fluviibacteracaefam。红环目11月。型应变为SHINM1T（=JCM 32071T=NCIMB 15105T）。
Three aerobic, Gram-stain-negative, non-motile, rod-shaped bacteria, designated as strains SHINM1T, ICHIJ1 and ICHIAU1, were isolated from surface river water (Saitama Prefecture, Japan). Phylogenetic analyses based on 16S rRNA and 40 marker gene sequences revealed that the strains formed a distinct phylogenetic lineage within the orderRhodocyclales. The three strains shared 100 % 16S rRNA gene similarity. Growth occurred at 15–30 °C and pH 6.0–9.5, but not in the presence of ≥1.0 % (w/v) NaCl. The isolates stained positive for intracellular polyphosphate granules. The major cellular fatty acids were C16 : 0, summed feature 2 (C12 : 1aldehyde and/or iso-C16 : 1I and/or C14 : 03-OH), summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c). The major polar lipids were phosphatidylethanolamine and an unidentified phospholipid. The predominant quinone system of strain SHINM1Twas ubiquinone-8 and its DNA G+C content was 56.7 mol%. Genome sequencing of the three isolates revealed a genome size of 2.29–2.43 Mbp and average nucleotide identity by orthology values of ≥98.9 %. Based on the results of phenotypic and phylogenetic analyses, strains SHINM1T, ICHIJ1 and ICHIAU1 represent a novel species of a new genus, for which the nameFluviibacter phosphoraccumulansgen. nov., sp. nov. is proposed, within a new family,Fluviibacteraceaefam. nov. of the orderRhodocyclales. The type strain is SHINM1T(=JCM 32071T=NCIMB 15105T).