High-throughput transcriptome sequencing reveals extremely high doses of ionizing radiation-response genes in Caenorhabditis elegans
High-throughput transcriptome sequencing reveals extremely high doses of ionizing radiation-response genes in Caenorhabditis elegans
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高通量转录组测序揭示了秀丽隐杆线虫中极高剂量的电离辐射反应基因
DOI：
10.1039/c9tx00101h
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发表时间：
2019
影响因子：
2.1
通讯作者：
Zhang Chao
中科院分区：
文献类型：
--
作者：
Xu Youqin;Chen Lina;Liu Mengyi;Lu Yanfang;Yue Yanwei;Liu Yue;Chen Honghao;Xie Fuliang;Zhang Chao
本研究在秀丽隐杆线虫（C.秀丽隐杆线虫）中寻找新的电离辐射反应（IR-response）基因。线虫). c。研究人员将线虫分为三组，分别接受0 Gy、200 Gy和400 Gy的高剂量辐照。提取各组总RNA并测序。当比较这些组之间的转录组时，发现许多基因存在差异表达，这些基因在ir相关的生物过程和途径中显著富集，包括与细胞行为、细胞生长和嘌呤代谢相关的基因本体（GO）术语，以及与ATP结合、GTPase调节活性和RNA降解相关的京都基因和基因组百科全书（KEGG）途径。定量反转录PCR （qRT-PCR）证实这些基因在不同处理中表现出差异表达。进一步的基因网络分析显示，鸟苷酸环化酶（GCY）、sm样蛋白（LSM）、二酰基甘油激酶（DGK）、skp1相关蛋白（SKR）和谷胱甘肽s转移酶（GST）基因家族表达上调。因此，这些基因可能在IR反应中发挥重要作用。同时，一些已知参与关键信号通路的重要基因，如磷酸肌醇特异性磷脂酶C-3 （PLC-3）、磷脂酰肌醇3-激酶age-1 （age-1）、Raf同源丝氨酸/苏氨酸蛋白激酶（LIN-45）和蛋白cbp-1 (cbp-1)，在IR反应中也出现了差异表达，表明IR反应可能干扰了这些关键信号通路。我们的研究揭示了秀丽隐杆线虫中一系列新的IR反应基因，这些基因可能作为IR反应的调节因子，并代表了有希望的IR暴露标记。
This study sought novel ionizing radiation-response (IR-response) genes inCaenorhabditis elegans(C. elegans).C. eleganswas divided into three groups and exposed to different high doses of IR: 0 gray (Gy), 200 Gy, and 400 Gy. Total RNA was extracted from each group and sequenced. When the transcriptomes were compared among these groups, many genes were shown to be differentially expressed, and these genes were significantly enriched in IR-related biological processes and pathways, including gene ontology (GO) terms related to cellular behaviours, cellular growth and purine metabolism and kyoto encyclopedia of genes and genomes (KEGG) pathways related to ATP binding, GTPase regulator activity, and RNA degradation. Quantitative reverse-transcription PCR (qRT-PCR) confirmed that these genes displayed differential expression across the treatments. Further gene network analysis showed a cluster of novel gene families, such as the guanylate cyclase (GCY), Sm-like protein (LSM), diacylglycerol kinase (DGK), skp1-related protein (SKR), and glutathione S-transferase (GST) gene families which were upregulated. Thus, these genes likely play important roles in IR response. Meanwhile, some important genes that are well known to be involved in key signalling pathways, such as phosphoinositide-specific phospholipase C-3 (PLC-3), phosphatidylinositol 3-kinase age-1 (AGE-1), Raf homolog serine/threonine-protein kinase (LIN-45) and protein cbp-1 (CBP-1), also showed differential expression during IR response, suggesting that IR response might perturb these key signalling pathways. Our study revealed a series of novel IR-response genes inCaenorhabditis elegansthat might act as regulators of IR response and represent promising markers of IR exposure.