Proteolipid protein from the peripheral nervous system also contains covalently bound fatty acids.
Proteolipid protein from the peripheral nervous system also contains covalently bound fatty acids.
复制标题
来自周围神经系统的蛋白脂质也含有共价结合的脂肪酸。
DOI：
10.1006/bbrc.1993.1767
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Bizzozero,OA
中科院分区：
文献类型：
--
作者：
Tetzloff,SU;Bizzozero,OA
Proteolipid protein, the major protein component of central nervous system (CNS) myelin, is known to contain approximately 2% by weight of covalently bound fatty acids. Recently, this protein was also found, though at a lower concentration, in the peripheral nervous system (PNS). The present study was undertaken to determine whether PNS proteolipid protein, like its CNS counterpart, was fatty acylated. Myeliri PLP and DM-20, the two proteins that constitute the proteolipid protein fraction, were isolated from bovine sciatic nerves by chromatography on a methylated Sephadex G-100 column. The identity of the isolated proteins was determined by (a) SDS-PAGE, (b) Western blot analysis, and (c) amino acid analysis, and fractions containing only PLP and DM-20 were subjected to acid-methanolysis. Gas-liquid chromatography of the released methyl esters revealed the presence of fatty acids (2.9% w/w) distributed as approximately 47% palmitic, 22% stearic, and 19% oleic acid. Similar results were obtained for bovine white matter proteolipid protein when isolated and analyzed in parallel with the peripheral nerve protein. These data unequivocally demonstrate that both PNS and CNS proteolipid protein are equally acylated.
