Expression of leptin receptors and potential effects of leptin on the cell growth and activation of mitogen-activated protein kinases in ovarian cancer cells
Expression of leptin receptors and potential effects of leptin on the cell growth and activation of mitogen-activated protein kinases in ovarian cancer cells
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DOI：
10.1210/jc.2004-0297
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发表时间：
2005-01-01
影响因子：
5.8
通讯作者：
Choi, KC
中科院分区：
文献类型：
--
作者：
Choi, JH;Park, SH;Choi, KC
瘦素是白色脂肪组织ob基因分泌的蛋白质，在大脑食物摄入和能量消耗的调节中发挥着重要作用，并且在正常和肿瘤性乳腺癌细胞中充当潜在的生长刺激剂。然而，瘦素作为内分泌调节剂在卵巢癌中的潜在作用尚不清楚。在本研究中，我们研究了永生化卵巢表面上皮（IOSE）和卵巢癌细胞系中瘦素受体的表达，以及瘦素对BG-1卵巢癌细胞系中细胞生长和丝裂原激活蛋白激酶（MAPK）激活的潜在影响。瘦素受体的短亚型和长亚型均在 IOSE-80PC（危机后细胞系）、BG-1、OVCAR-3 和 SKOV-3 细胞中表达。此外，用瘦素处理会刺激 BG-1 细胞的生长、激活 ERK1/2 并抑制 p38 MAPK 的组成型磷酸化。这些结果表明，需要进一步的研究来验证瘦素是否可能是卵巢癌的潜在调节剂。
Leptin a secreted protein of the ob gene by white adipose tissue, plays an important role in the regulation of food intake and energy consumption in the brain and acts as a potential growth stimulator in normal and neoplastic breast cancer cells. However, a potential role of leptin as an endocrine regulator is unknown in ovarian cancer. In the present study, we investigated the expression of leptin receptors in immortalized ovarian surface epithelium (IOSE) and ovarian cancer cell lines, and potential effect of leptin on the cell growth and activation of mitogen-activated protein kinases (MAPKs) in the BG-1 ovarian cancer cell line. Both short and long isoforms of leptin receptors are expressed in IOSE-80PC (a post-crisis line), BG-1 OVCAR-3 and SKOV-3 cells. In addition, treatment with leptin resulted in the growth stimulation of BG-1 cells, an activation of ERK1/2 and inhibition of constitutive phosphorylation of p38 MAPK. These results suggest that further studies are necessary to validate whether leptin may be a potential regulator for ovarian cancer.